C5ar2 (NM_001146005) Mouse Untagged Clone

CAT#: MC214409

C5ar2 (untagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 2, (10ug)


  "NM_001146005" in other vectors (4)

Reconstitution Protocol

USD 503.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol C5ar2
Synonyms C5L2; E030029A11Rik; Gpr77
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC214409 representing NM_001146005
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGGCAGAGAACAGCGCAGCCGGTGTCTGGGGGCCAGAATGATGAACCACACCACCAGCGAGTATT
ATGACTATGAGTATGACCATGAACACTACAGTGACCTTCCGGACGTGCCCGTAGACTGCCCAGCTGGCAC
CTGCTTCACTAGTGATGTCTACCTGATAGTCCTGCTTGTGCTGTATGCAGCCGTCTTCCTGGTGGGTGTG
CCAGGCAACACCCTGGTGGCGTGGGTGACCTGGAAAGAGTCCCGCCACAGGCTTGGGGCCTCTTGGTTCC
TGCACCTGACCATGGCCGACTTGCTTTGTTGTGTGTCTCTGCCCTTCCTGGCTGTGCCCATCGCCCAAAA
GGGCCACTGGCCATACGGGGCGGCAGGCTGCTGGCTGTTGTCCTCCATCACCATTCTGTCTATGTATGCC
AGTGTGCTGCTCCTGACCGGCCTCAGCGGAGACCTCTTCCTACTGGCTTTCAGGCCCTCCTGGAAGGGTG
CAGATCATCGAACATTTGGGGTGCGTGTGGTCCAGGCCTCCTCCTGGATGCTGGGCCTCTTGCTGACTGT
GCCTTCTGCTGTCTACCGTAGGCTGCTTCAGGAGCACTATCCTCCCCGACTTGTGTGTGGCATAGACTAT
GGGGGATCGGTCAGCGCCGAGGTTGCCATCACCACTGTTCGATTCCTCTTTGGCTTCCTGGGGCCATTGG
TGTTCATGGCCGGTTGTCACGGCATCCTCCAACGGCAGATGGCCCGCCGCCATTGGCCCCTGGGCACAGC
TGTCGTGGTGGGGTTTTTCATTTGCTGGACCCCTTATCACGTCCTGCGGGTAATCATTGCAGCGGCTCCT
CCGCACTCCTTGCTCCTCGCCCGGGTCCTGGAGGCGGAGCCTCTGTTTAATGGCCTGGCCCTGGCTCACA
GTGCTCTCAATCCCATAATGTTTTTGTATTTCGGAAGAAAACAACTCTGCAAGTCACTCCAGGCTGCATG
CCACTGGGCCCTGAGGGATCCACAGGATGAGGAAAGTGCGGTGACCAAGGTATCCATATCCACCAGCCAC
GAAATGGTGTCTGAGATGCCGGTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001146005
Insert Size 1077 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001146005.1, NP_001139477.1
RefSeq Size 3429 bp
RefSeq ORF 1077 bp
Locus ID 319430
UniProt ID Q8BW93
Cytogenetics 7 A2
Gene Summary Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a distinct N-terminus but is the same length as isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.