Mpst (NM_001162492) Mouse Untagged Clone

CAT#: MC213244

Mpst (untagged) - Mouse mercaptopyruvate sulfurtransferase (Mpst), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mpst
• Synonyms: Mst
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC213244 representing NM_001162492
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCCCGCAACTTTTCCGAGCTCTGGTGTCTGCGCAGTGGGTGGCGGAGGCTCTGAAGGCCCCGC
  GCTCCTCACAGCCGCTGAAGTTACTGGACGCGTCCTGGTACCTACCCAAGCTGGGCCGCGATGCACGACG
  CGAGTTTGAGGAGCGCCACATCCCGGGCGCCGCCTTCTTCGACATCGATCGTTGCAGTGACCACACGTCC
  CCCTACGACCACATGCTGCCTAACGCCACGCACTTTGCCGACTACGCGGGCAGCTTGGGCGTGAGCGCCG
  CCACGCACGTCGTGATCTACGACGACAGTGACCAGGGCCTCTATTCCGCTCCGCGGGTCTGGTGGATGTT
  CCGCGCCTTCGGCCACCACTCTGTGTCATTGCTGGACGGCGGCTTCCGCCACTGGCTGAATCAGAACCTG
  CCAATCAGCTCCGGCAAAAGCCACTCGGAGCCCGCGGAGTTCAGCGCGCAGCTCGATCCCTCTTTCATCA
  AGACCCACGAGGACATCCTGGAGAACTTGGATGCCCGGCGCTTCCAGGTGGTGGACGCCCGCGCAGCTGG
  CCGTTTCCAAGGCACCCAGCCAGAACCCCGAGATGGCATCGAACCTGGCCACATCCCTGGCTCAGTAAAC
  ATCCCGTTCACTGAATTCCTGACCAACGAAGGCCTGGAGAAGAGCCCAGAGGAGATCAAACGCCTGTTTA
  AGGAGAAGAAGGTGGACCTGTCTAAGCCCTTGGTAGCCACGTGTGGCTCCGGTGTCACAGCCTGCCACGT
  GGTCCTGGGGGCCTTCCTCTGTGGCAAGTCAGACGTGCCTGTCTATGATGGCTCCTGGGTAGAGTGGTAC
  ATGCGTGCCCAGCCAGAGCATATCATCTCTGAGGGCCGAGGGAAGACCCAGTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001162492
• Insert Size: 894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162492.1, NP_001155964.1
• RefSeq Size: 1322 bp
• RefSeq ORF: 894 bp
• Locus ID: 246221
• UniProt ID: Q99J99
• Cytogenetics: 15 E1
• Gene Summary: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.