Psd3 (NM_027626) Mouse Untagged Clone

CAT#: MC212984

Psd3 (untagged) - Mouse pleckstrin and Sec7 domain containing 3 (Psd3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Psd3
• Synonyms: 4931420C21Rik; AI661273; BC003498; D430018P08; EFA6D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212984 representing NM_027626
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGGCGTGAATAGTGTTCAGAGTGCCAGCAAGGTACGGGTGAGGACCTCAGGTTCTGTGAAGTACT
  CCCGAGAGGATTCTATCCGGCGGCAGTCCCACCGGTCAAAGTCTATGAAAATTTCCAACTCCTCAGAGTT
  TTCTGCTAAGGAGAGCAAGGCTCTGTACAACTCAATCAAGAACGAGAAGCTTGAATGGGCAGTAGATGAT
  GAAGAGAAAAAAAAATCCCCCTCAGAAGGTACTGATGAGAAGGCTAATGGAACGCACCCAAAGACTATCA
  GTCGTATTGGGAGTACAACGAACCCATTTTTGGACATTCCTCATGACCCGAATGCTGCTGTATACAAAAG
  TGGATTTTTGGCTCGTAAAATCCACGCAGACATGGATGGAAAGAAGACTCCAAGAGGAAAGCGAGGATGG
  AAAACCTTTTATGCTGTCCTGAAGGGAACCGTTCTTTACTTACAGAAGGATGAGTATAAGCCAGAGAAGT
  CTCTGTCCGATGAAGACTTGAAGAATGCGGTGAGTGTGCACCACGCTCTGGCGTCCAAGGCCACTGACTA
  TGAGAAGAAGCCGAACGTGTTTAAACTCAAGACTGCGGACTGGAGGGTCTTGCTTTTCCAAACCCAGAGT
  CCAGAGGAAATGCAAGGCTGGATAAACAAAATTAACTGTGTGGCAGCTGTGTTTTCTGCACCGCCTTTCC
  CAGCAGCCATTGGCTCTCAGAAGAAGTTTAGCCGCCCACTTCTGCCAGCCACTACAACAAAATTATCCCA
  GGAGGAGCAGCTGAAGTCCCATGAAAGCAAGCTGAAGCAGATCACCACTGAGCTGGCTGAGCATCGCTCA
  TATCCCCCAGACAAGAAGGTCAAAGCCAAGGATGTTGATGAGTACAAGCTGAAAGACCACTATCTGGAGT
  TCGAGAAAACCCGTTATGAGATTTATGTCAGCGTTCTGAAAGAAGGAGGCAAAGAGCTCCTGACTACCGA
  TGGAAATGAGCCCGTGGGACTGAAGAAGTCACACTCAAGTCCCTCCCTCAACCCAGATGCATCTCCAGTC
  ACTGCCAAGGTCAAGCGCAACGTGTCAGAGAGAAAAGACCACCGACCTGAAACACCGGGCATTAAGCAAA
  AAGTTACTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027626
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027626.1, NP_081902.1
• RefSeq Size: 9228 bp
• RefSeq ORF: 1131 bp
• Locus ID: 234353
• UniProt ID: Q2PFD7
• Cytogenetics: 8 B3.2- B3.3
• Gene Summary: Guanine nucleotide exchange factor for ARF6.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks several 5' exons but contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation from an alternate start codon, compared to variant 2. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 2.