B3gnt4 (NM_198611) Mouse Untagged Clone

CAT#: MC212926

B3gnt4 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 (B3gnt4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: B3gnt4
• Synonyms: 1010001G17Rik; BGnT-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212926 representing NM_198611
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCAGGTTGGGCTGTGTGCTGTTCTGCAGTCTTGTGGTACTACTGCTCAGCTGCCTGCTCCTTC
  TGAAGGAGCGCATACCAGCAGGCAGCTCCAAGGCCCACCAGCAGTTCTTGGCACTGCCAAGGTCCCACCA
  TAGCCAGTGCTCACCCAACCTAACAGTTGTTAACACCTCCCTGTCCCTGCCCAGCCGTCATCGCCTCTTC
  TTAACCTATCGACACTGCCGAAACTTCTCCATCTTGCTGGAGCCTTCGGAGTGTGCCAGGGACACCTTCC
  TGCTCCTGGTCATCAAGTCACAGCCTGCTCATATTGAACAGCGTTCAGCCATTCGAAGCACTTGGGGCCG
  GGCTGGGAGCTGGGCTAGGGGTCGGCAGCTGAAGCTGGTGTTCCTCCTGGGGGTGGCAGGACCTGTGCCC
  CCGGCCCAGCTGTTGGTCTATGAGAGCTGGCAGTTTGATGACATCTTACAGTGGGACTTTGCTGAGGACT
  TCTTTAACCTGACTCTCAAGGAGCTGCACGTGCAGCGCTGGATCGCAGCTGCCTGCACACAGGCCCACTT
  CATACTAAAGGGAGACGATGACGTCTTTATCCATGTTCCCAATGTGCTCGAGTTCTTGGAGGGCTGGGAT
  CCGGCCCAGGACTTCCTAGTGGGAGATGTCATTCGCTTGGCCCGGCCCAACAGGAACACCAAAGTTAAAT
  ATTTCATCCCGTTTTCCATGTACAGGGCCCGCCACTACCCACCTTATGCAGGAGGCGGCGGCTACGTCAT
  GTCCCAGGCTACTGTGAGGCATCTTCACATGGCCATGGAGGAGGCAGAGCTCTTTCCTATTGACGATGTC
  TTTGTGGGGATGTGCCTGAGGAAGCTGGGGGTGACTCCCATACACCATGCTGGCTTCAAGACATTTGGAA
  TCCAACAGCCCCTCAATCCCAGGGACCCTTGTCTGTACAAAGGGCTCCTGCTGGTGCACCGTCTCAGCCC
  CCTGGAGATGTGGACCATGTGGGCGCTGGTGACAGATGAGAGGCTTAAGTGTGCAGCTACCCACAAGCCC
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198611
• Insert Size: 1053 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198611.2, NP_941013.2
• RefSeq Size: 1423 bp
• RefSeq ORF: 1053 bp
• Locus ID: 231727
• UniProt ID: Q1RLK6
• Cytogenetics: 5 F
• Gene Summary: Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides.[UniProtKB/Swiss-Prot Function]