P2rx2 (NM_001164833) Mouse Untagged Clone

CAT#: MC212923

P2rx2 (untagged) - Mouse purinergic receptor P2X, ligand-gated ion channel, 2 (P2rx2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P2rx2
• Synonyms: P2x2; P2X2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212923 representing NM_001164833
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGCACAGCCCCGGCTTCCCGCGGGGGCGGCCATGGTCCGGCGCTTGGCCCGGGGCTGCTGGT
  CCGCGTTCTGGGACTACGAGACGCCCAAGGTGATCGTGGTGCGGAATCGGCGCCTGGGATTCGTGCACCG
  CATGGTGCAGCTGCTCATTCTGCTTTACTTCGTGTGGTACGTCTTCATCGTGCAGAAAAGCTACCAGGAT
  AGCGAAACCGGTCCGGAGAGCTCCATCATCACCAAAGTCAAGGGGATCACCATGTCGGAACACAAAGTGT
  GGGACGTGGAGGAATACGTAAAGCCCCCGGAGGGGGGCAGTGTAGTCAGCATCATCACCAGGATCGAGGT
  TACTCCTTCCCAGACCCTGGGAACATGCCCAGAGAGCATGAGGGTTCACAGCTCTACCTGCCATTTAGAT
  GACGACTGTGTGGCCGGACAGCTGGACATGCAGGGCAATGGGATTCGGACAGGACGCTGTGTACCCTATT
  ACCATGGGGACTCCAAGACCTGCGAGGTGTCAGCCTGGTGCCCGGTGGAGGATGGGACTTCTGAAAACCA
  TTTTCTGGGTAAAATGGCCCCAAATTTCACCATCCTCATCAAGAACAGCATCCACTATCCCAAGTTCAAG
  TTCTCCAAGGGCAACATTGCAAGCCAGAAGAGTGACTACCTGAAGCACTGCACGTTTGATCAGGACTCTG
  ATCCATACTGTCCCATCTTCAGGCTGGGCTTCATTGTAGAGCAAGCAGGAGAGAACTTCACAGAACTGGC
  ACACAAGGGCGGTGTCATTGGGGTCATCATCAACTGGAACTGTGACCTGGACTTGTCTGAATCAGAGTGC
  AACCCCAAATATTCTTTCCGGAGGCTCGACCCCAAGTATGACCCTGCCTCTTCAGGCTACAACTTCAGGT
  TTGCCAAATATTACAAGATAAACGGCACCACCACCACTCGAACTCTCATCAAAGCCTATGGGATTCGAAT
  TGACGTTATTGTGCATGGACAGGCAGGGAAATTCAGTCTCATTCCCACCATCATCAATCTGGCCACTGCT
  CTGACCTCCATCGGGGTGGGCTCCTTTCTGTGTGACTGGATTTTGTTAACGTTCATGAACAAAAACAAGC
  TCTACAGCCATAAGAAGTTCGACAAGGTGGTGGACACTCTTGACCAGCATATGGGACAAAGGCCTCCTGT
  CCCTGAGCCTTCCCAACAGGACTCCACATCCACGGACCCCAAAGGTTTGGCCCAACTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001164833
• Insert Size: 1251 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164833.1, NP_001158305.1
• RefSeq Size: 1712 bp
• RefSeq ORF: 1251 bp
• Locus ID: 231602
• UniProt ID: Q8K3P1
• Cytogenetics: 5 F
• Gene Summary: Ion channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame segment in the 3' coding region compared to variant 1. The encoded protein (isoform b) is shorter than isoform a.