Insig1 (NM_153526) Mouse Untagged Clone

CAT#: MC212914

Insig1 (untagged) - Mouse insulin induced gene 1 (Insig1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Insig1
• Synonyms: 1810013C12Rik; Insig-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212914 representing NM_153526
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGGCTGCACGACCACGTCTGGAACTATCCAAGCGCGGGCGCTGCGAGGCCGTACAGCCTCCCGC
  GAGGCATGATTGCGGCGGCCGCTTGTCCGCAGGGCCCCGGAGTCCCCGAACCCGAGCACGCGCCCCGGGG
  CCAGCGAGCGGGAACCACAGGATGCAGCGCGCGGCCGGGCAGCTGGCACCACGACTTGGTGCAGCGGAGC
  CTAGTGCTCTTCTCATTTGGCGTGGTCCTGGCCCTGGTGCTCAACCTGCTGCAGATCCAGCGGAATGTCA
  CGCTCTTCCCCGACGAGGTGATAGCCACCATCTTCTCCTCCGCCTGGTGGGTGCCCCCGTGCTGTGGCAC
  GGCAGCGGCTGTTGTCGGTTTACTGTATCCCTGTATTGACAGTCACCTGGGAGAACCACACAAGTTCAAG
  AGAGAATGGGCCAGCGTTATGCGCTGTATTGCCGTGTTCGTTGGCATCAACCACGCCAGTGCCAAATTAG
  ATTTTGCCAATAATGTCCAGCTGTCCTTGACTTTAGCAGCCCTCTCTTTGGGTTTGTGGTGGACATTTGA
  TCGTTCCCGAAGCGGCCTTGGGCTTGGGATTACCATCGCCTTCCTAGCTACGCTGATCACTCAGTTTCTT
  GTGTATAATGGTGTCTACCAGTACACGTCCCCTGATTTCCTCTATATCCGTTCTTGGCTCCCCTGTATAT
  TTTTCTCAGGAGGTGTCACAGTGGGAAACATAGGACGACAGTTAGCTATGGGTGTTCCGGAAAAGCCTCA
  CAGTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153526
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153526.5, NP_705746.1
• RefSeq Size: 2667 bp
• RefSeq ORF: 780 bp
• Locus ID: 231070
• UniProt ID: Q8BGI3
• Cytogenetics: 5 B1
• Gene Summary: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.[UniProtKB/Swiss-Prot Function]