Acer2 (NM_139306) Mouse Untagged Clone

CAT#: MC212879

Acer2 (untagged) - Mouse alkaline ceramidase 2 (Acer2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acer2
• Synonyms: 2410116I05Rik; Asah3l; CRG-L1; maCER2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212879 representing NM_139306
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCCCGCACTGGTGGGACCACCTGCGGGCTGGCAGTTCGGAGGTGGATTGGTGCGAGGACAACT
  ACACTATCGTGCCTGCCATTGCCGAGTTCTACAACACGATCAGCAACGTCTTGTTTTTCATTTTACCTCC
  CATCTGCATGTGCTTGTTCCGCCAGTACGCAACGTGCTTCAACAGCGGCATCTACTTAATATGGACGCTC
  CTAGTTGTAGTGGGGATTGGATCTGTCTACTTCCATGCAACGCTGAGTTTCCTGGGTCAGATGCTTGATG
  AACTTGCCATTCTGTGGGTTCTGATGTGTGCTTTGGCCATGTGGTTTCCCAGGAGGTATTTACCAAAGAT
  CTTTCGGAATGACAGGGGCAGGTTCAAGGCAGTGGTGTGTGTCCTGTCTGCAATTACAACGTGCTTGGCG
  TTTATCAAGCCCGCCATCAACAATATTTCCCTGATGATTCTGGGACTTCCATGCACTGCGCTGCTTGTTG
  CAGAGCTGAAGAGGTGTGACAATGTGCGTGTGTTTAAGCTGGGCCTCTTCTCTGGCCTCTGGTGGACTCT
  GGCTCTCTTCTGCTGGATCAGCGACCAAGCCTTCTGTGAGCTGCTCTCCTCCTTTCACTTCCCCTACCTG
  CACTGTGTGTGGCATATTCTCATCTGCCTTGCTTCGTACCTGGGCTGTGTGTGCTTCGCCTACTTTGATG
  CTGCCTCAGAGATACCTGAGCAAGGTCCAGTCATCAGATTCTGGCCCAGCGAGAAATGGGCTTTTATTGG
  TGTCCCTTATGTGTCCCTTCTGTGTGCCCACAAGAAGTCGCCAGTCAAGATCACGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_139306
• Insert Size: 828 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139306.3, NP_647467.1
• RefSeq Size: 4206 bp
• RefSeq ORF: 828 bp
• Locus ID: 230379
• UniProt ID: Q8VD53
• Cytogenetics: 4 C4
• Gene Summary: Golgi ceramidase that catalyzes the hydrolysis of ceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:29401619). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:29401619). Has a better catalytic efficiency towards unsaturated long-chain ceramides, including C18:1-, C20:1- and C24:1-ceramides (By similarity) (PubMed:29401619). Saturated long-chain ceramides and unsaturated very long-chain ceramides are also good substrates, whereas saturated very long-chain ceramides and short-chain ceramides are poor substrates. Also hydrolyzes dihydroceramides to produce dihydrosphingosine (By similarity). It is the ceramidase that controls the levels of circulating sphingosine-1-phosphate and dihydrosphingosine-1-phosphate in plasma through their production by hematopoietic cells (PubMed:29401619). Regulates cell proliferation, autophagy and apoptosis by the production of sphingosine and sphingosine-1-phosphate. As part of a p53/TP53-dependent pathway, promotes for instance autophagy and apoptosis in response to DNA damage. Through the production of sphingosine, may also regulate the function of the Golgi complex and regulate the glycosylation of proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).