Cdnf (NM_177647) Mouse Untagged Clone

CAT#: MC212801

Cdnf (untagged) - Mouse cerebral dopamine neurotrophic factor (Cdnf), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdnf
• Synonyms: 9330140G23; Armetl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212801 representing NM_177647
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTGCATCAGTCCAACTGCTCTGGTGACCTTTTGCGCCGGGTTTTGTATCTCGAACCCTGTGCTGG
  CGCAGGGCCTGGAGGCCGGTGTGGGGCCGAGGGCTGACTGTGAAGTATGTAAAGAATTCTTAGACCGATT
  CTACAACTCCCTGCTAAGCAGAGGCATAGACTTTTCTGCGGACACCATAGAGAAAGAGCTGCTCAACTTT
  TGCTCAGATGCCAAAGGAAAAGAAAACCGCCTGTGCTATTATCTGGGGGCCACCACAGATGCAGCCACCA
  AGATCCTAGGAGAAGTCACTCGTCCCATGAGTGTACACATACCTGCCGTGAAGATTTGTGAGAAGCTAAA
  GAAGATGGACAGCCAGATCTGTGAGCTGAAATACGGGAAGAAGCTGGACTTGGCGTCGGTGGACCTGTGG
  AAGATGAGAGTGGCAGAGCTAAAGCAGATCCTTCAGAGATGGGGGGAAGAGTGCAGGGCATGTGCGGAGA
  AAAGTGACTACGTGAACCTCATTAGAGAGCTGGCCCCCAAATATGTAGAGATATACCCCCAAACGGAGCT
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177647
• Insert Size: 564 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177647.4, NP_808315.1
• RefSeq Size: 3013 bp
• RefSeq ORF: 564 bp
• Locus ID: 227526
• UniProt ID: Q8CC36
• Cytogenetics: 2 A1
• Gene Summary: Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).[UniProtKB/Swiss-Prot Function]