Gpbar1 (NM_174985) Mouse Untagged Clone

CAT#: MC212798

Gpbar1 (untagged) - Mouse G protein-coupled bile acid receptor 1 (Gpbar1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpbar1
• Synonyms: BG37; GPCR; GPR131; M-BAR; TGR5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_174985.1
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACACCCAACAGCACTGAGCTGTCGGCCATTCCCATGGGGGTTCTGGGGCTTTCCTTGGCCCTGG
  CAAGCCTCATCGTCATCGCCAACCTGCTCCTGGCCCTAGGCATCGCCCTGGACCGCCACTTGCGCAGCCC
  ACCTGCTGGCTGCTTCTTCCTAAGCCTACTACTAGCCGGGCTGCTCACAGGGCTGGCACTGCCCATGCTG
  CCTGGGCTATGGAGCCGGAACCATCAGGGCTACTGGTCCTGCCTCCTTCTCCACTTGACCCCCAACTTTT
  GTTTCCTTTCCCTGCTTGCCAATCTGCTGCTGGTGCATGGGGAACGCTACATGGCAGTGTTGCAGCCACT
  CCGGCCCCATGGAAGTGTGCGGCTAGCCCTGTTCCTCACCTGGGTCAGCTCCCTGTTCTTTGCCAGCCTG
  CCTGCTCTGGGCTGGAACCATTGGAGCCCTGATGCCAACTGCAGCTCCCAAGCTGTCTTCCCAGCCCCCT
  ACCTCTACCTGGAAGTTTATGGCCTCCTGTTGCCTGCCGTGGGGGCCACTGCCCTTCTCTCTGTCCGCGT
  GTTGGCCACTGCCCACCGCCAGCTGTGTGAGATCCGCCGACTGGAGCGGGCAGTGTGCCGCGATGTACCC
  TCAACCCTGGCTAGGGCTCTCACCTGGAGGCAGGCTAGGGCACAGGCAGGAGCCACACTGCTCTTCTTGC
  TGTGTTGGGGGCCCTATGTGGCCACATTGCTCCTGTCAGTCTTGGCCTATGAGCGTCGCCCACCACTAGG
  GCCTGGAACTCTGTTATCGCTCATCTCATTGGGCAGCACCAGTGCTGCCGCTGTGCCTGTGGCCATGGGG
  CTGGGTGATCAGCGCTACACAGCCCCCTGGAGGACAGCTGCCCAAAGGTGTCTACGAGTGCTTCGAGGAA
  GAGCCAAGAGGGACAATCCAGGCCCCAGCACTGCCTACCACACCAGTAGCCAATGCAGCATTGACCTGGA
  CTTGAATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_174985
• Insert Size: 990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174985.1, NP_778150.1
• RefSeq Size: 990 bp
• RefSeq ORF: 990 bp
• Locus ID: 227289
• UniProt ID: Q80SS6
• Cytogenetics: 1 38.53 cM
• Gene Summary: Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids (By similarity). Involved in bile acid promoted GLP1R secretion.[UniProtKB/Swiss-Prot Function]