Nmnat2 (NM_175460) Mouse Untagged Clone

CAT#: MC212784

Nmnat2 (untagged) - Mouse nicotinamide nucleotide adenylyltransferase 2 (Nmnat2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nmnat2
• Synonyms: AI843915; D030041I09Rik; PNAT1; PNAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_175460, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCGAGACCACAAAGACCCACGTTATCCTGCTGGCCTGCGGCAGCTTCAATCCCATCACTAAAGGGC
  ACATTCAGATGTTCGAGAGAGCCAGGGATTATCTGCACAAGACTGGAAGATTTATTGTGATTGGCGGGAT
  TGTCTCTCCGGTCCATGACTCCTACGGAAAACAGGGCCTTGTGTCAAGTCGGCACCGTCTCATCATGTGT
  CAGCTGGCTGTCCAGAATTCCGACTGGATCAGGGTGGACCCATGGGAGTGCTATCAGGACACCTGGCAGA
  CAACCTGCAGTGTGTTGGAGCACCATCGAGACCTGATGAAGAGGGTGACCGGCTGCATCCTCTCCAACGT
  CAACACACCTTCCATGACACCTGTGATCGGACAGCCACAGCATGAGAACACCCAGCCCATTTACCAGAAC
  AGCAATGTGCCCACCAAGCCCACTGCAGCCAAGATCTTGGGAAAGGTGGGAGAAAGCCTCAGCCGGATCT
  GCTGCGTCCGCCCACCAGTGGAGCGCTTCACTTTTGTAGATGAGAACGCCAACCTGGGCACAGTGATGCG
  GTATGAGGAGATCGAGCTGCGCATCTTGCTGCTGTGTGGTAGTGACCTGCTGGAGTCTTTCTGCATCCCA
  GGACTCTGGAATGAGGCAGATATGGAAGTGATTGTTGGGGACTTTGGGATCGTCGTGGTACCCAGGGATG
  CAGCGGACACAGACCGGATCATGAATCACTCCTCCATACTCCGCAAGTACAAAAACAACATCATGGTTGT
  GAAGGATGATATCAACCATCCCATGTCTGTAGTCAGCTCCACCAAGAGCAGGCTGGCCCTGCAGCATGGG
  GACGGCCATGTTGTGGATTACCTGTCCCAGCCAGTCATCGATTACATCCTCAAGAGTCAGCTGTACATCA
  ACGCCTCGGGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_175460
• Insert Size: 924 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC089007, AAH89007
• RefSeq Size: 4548 bp
• RefSeq ORF: 924 bp
• Locus ID: 226518
• UniProt ID: Q8BNJ3
• Cytogenetics: 1 G3
• Gene Summary: Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor (PubMed:20126265, PubMed:23082226). Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (By similarity). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency (By similarity). Cannot use triazofurin monophosphate (TrMP) as substrate (By similarity). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+) (By similarity). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively (By similarity). Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+) (By similarity). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons (PubMed:20126265, PubMed:23082226).[UniProtKB/Swiss-Prot Function]