Kmt5b (NM_001167889) Mouse Untagged Clone

CAT#: MC212766

Suv420h1 (untagged) - Mouse suppressor of variegation 4-20 homolog 1 (Drosophila) (Suv420h1), transcript variant 5, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kmt5b
• Synonyms: AA117471; C630029K18Rik; Suv4-20h1; Suv420h1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212766 representing NM_001167889
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGTTGGGAGACTCCAAGAACATGGTGGTGAATGGCAGGAGAAATGGAGGCAAGTTGTCTAATG
  ACCATCAGCAGAATCAATCAAAATTACAGCAGCACTCGGGCAAGGACACCCTGAAGACCGGCAGAAACGC
  CGTTGAGAGGCGGTCCAGCAGATGTCATGGTAACTCGGGATTTGAAGGGCAGAGCCGCTATGTGCCGTCC
  TCTGGAATGTCCGCCAAGGAGCTCTGTGAGAACGATGACTTAGCAACCAGTTTGGTTCTTGATCCCTACT
  TAGGTTTTCAGACACACAAAATGAACACTAGCGCCTTTCCTTCGAGGAGCTCGAGGCATATTTCAAAAGC
  TGACAGTTTTTCTCACAACAATCCTGTGAGATTTCGGCCTATAAAAGGAAGGCAAGAAGAGCTAAAGGAA
  GTAATTGAACGCTTTAAGAAAGATGAACACTTAGAGAAAGCTTTCAAATGTTTGACTTCTGGGGAATGGG
  CACGGCATTATTTTCTCAACAAAAACAAAATGCAGGAGAAATTATTCAAGGAACATGTCTTTATTTACTT
  GCGGATGTTTGCAACTGACAGTGGATTTGAAATACTGCCTTGTAATAGATATTCTTCAGAACAAAATGGA
  GCCAAGATAGTTGCAACAAAAGAGTGGAAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCCG
  AACTTTCAGAAATTGAGGAGAACATGCTACTTAGACACGGAGAAAACGACTTCAGTGTCATGTATTCCAC
  AAGGAAAAATTGTGCTCAACTCTGGCTCGGTCCTGCTGCATTTATAAATCATGATTGCAGACCTAACTGT
  AAGTTTGTGTCAACTGGTCGAGATACAGCATGCGTTAAGGCTCTGAGAGATATTGAACCTGGAGAAGAAA
  TTTCTTGTTACTATGGAGATGGCTTTTTTGGAGAAAATAATGAGTTCTGCGAATGTTATACTTGTGAAAG
  ACGGGGAACTGGTGCTTTTAAATCACGAGTAGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATAC
  GGACTTAGAGAAACAGATAAACGCTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAACTCAGACA
  GTCAGTCTGTCAGCTCTAACACAGATGCAGACACCACTCAGGAAAAAGACAATGCAAGTAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167889
• Insert Size: 1185 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167889.1, NP_001161361.1
• RefSeq Size: 3290 bp
• RefSeq ORF: 1185 bp
• Locus ID: 225888
• UniProt ID: Q3U8K7
• Cytogenetics: 19 A
• Gene Summary: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) differs in the 5' UTR, the 3' UTR, and the 3' coding sequence compared to variant 1. The resulting isoform (c) has a shorter and distinct C-terminus compared to isoform a.