Lclat1 (NM_001177968) Mouse Untagged Clone

CAT#: MC212744

Lclat1 (untagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lclat1
• Synonyms: Agpat8; AI181996; Alcat1; Gm91; Lycat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212744 representing NM_001177968
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCATGGAAGGGGATTTACTTTATACTCTTCCTGTTTGCTGGAAGCTTTTTTGGAAGTATTTTTA
  TGCTCGGCCCCATTTTACCTTTGATGTTTATAAACCTGTCGTGGTATCGCTGGATTAGCAGCCGTCTTGT
  GGCTACATGGCTCACACTTCCTGTGGCATTGCTGGAGACCATGTTTGGTGTGAGAGTGGTTATAACAGGT
  GACGCCTTTGTGCCTGGAGAGCGGAGCGTCATCATCATGAACCACCGGACACGTGTGGACTGGATGTTCC
  TGTGGAACTGTCTAATGAGGTACAGCTACCTCAGGGTGGAGAAGATTTGCCTCAAATCCAGTCTCAAAAG
  TGTTCCTGGATTCGGCTGGGCCATGCAAGTTGCGGCCTTTATCTTTATTCATAGGAAGTGGAAGGATGAT
  AAGAGCCATTTTGAAGACATGATTGATTATTTTTGTGCCATCCATGAACCACTACAGCTTCTCATTTTTC
  CAGAAGGAACTGACCTCACAGAAAATAATAAGGCTAGGAGTAATGATTTTGCTGAGAAGAACGGACTTCA
  GAAATATGAGTATGTTTTACACCCAAGAACCACTGGCTTTACCTTTGTGGTGGACCGCCTAAGAGAAGGG
  AAGAACCTCGATGCTGTTCATGACATCACGGTCGCATATCCTTACAACATCCCTCAAACTGAGAAGCACC
  TTCTCCTTGGAGACTTTCCCAAGGAGATCCACTTCCACGTCCAGCGGTATCCAGCTGACTCTCTTCCCAC
  ATCCAAGGAGGACCTTCAGCTCTGGTGCCACAGAAGGTGGGAAGAAAAGGAGGAGAGGCTTCGGTCCTTC
  TACCAAGGAGAGAAAAACTTCCACTTTACTGGGCAGAGTACAGTTCCACCTTGCAAGTCTGAGCTCAGAG
  TCCTTGTGGTCAAGCTACTGTCCATAGTGTACTGGGCCTTGTTCTGCTCTGCAATGTGCCTGCTCATATA
  TCTGTACAGCCCTGTTCGGTGGTATTTTATAATCAGCATTGTGTTCTTCGTGCTGCAGGAGAGAATATTT
  GGTGGACTGGAAATCATTGAACTTGCATGTTACCGTTTTTTACACAAGCACCCACATTTAAATTCAAAGA
  AAAATGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177968
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177968.1, NP_001171439.1
• RefSeq Size: 4444 bp
• RefSeq ORF: 1131 bp
• Locus ID: 225010
• UniProt ID: Q3UN02
• Cytogenetics: 17 E1.3- E2
• Gene Summary: Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (PubMed:15152008). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (PubMed:15152008). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Possesses lysophosphatidylinositol acyltransferase (LPIAT) activity (PubMed:20668164). Possesses lysophosphatidylglycerol acyltransferase (LPGAT) activity (By similarity). Required for establishment of the hematopoietic and endothelial lineages (PubMed:17675553).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.