H2-M10.5 (NM_177637) Mouse Untagged Clone

CAT#: MC212736

H2 (untagged) - Mouse histocompatibility 2, M region locus 10.5 (H2-M10.5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: H2-M10.5
• Synonyms: 6.9H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212736 representing NM_177637
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAACCCTGGATGCTGCACACTCCTCCTCCTCTTGGTGGCCATGGACCTGATCCAGTATTGTGCAG
  GCTCACACTGGCTGCAGACTTTCAACGCTGTGATTTTGGAGCCTGGCATGGTTAATTCCCGGTTCGTCCA
  CATTGGCTATGTAGACTCTATAGAGTATCAGAGATATGACAGCAAAGAACCAATTGCAGTTTTGCTACCT
  CGGGTTGCATGGATGGAGCAGGTGCCAATGACATATTGGACCAGTGAGACAGCAAGCGTTGCGGAATTGT
  CACAGCTAGACAGACGAATTCTTCACTTCATGGTGAACAAAAATGAACAAAGAATGGACGATTATCACAC
  CCTGCAGGAAGTGTATGGCTGCAATGTGGCTAATGATGGGAGCTTCCTCGGCGGTCACTTCCGGCTCACC
  TACTATGGCTATGATGACTTATACCTGAATGAGAACCTGAGCTCCTGGATTGCACATGGAAACGCAGCTG
  AATATGTGAAGAGCAGGTGGGACGGTGAAGGTGATGCAGAAAGGTGGAAGACTTACCTGCAAGGAGTGTG
  CGTGGAAAGGCTTCTTAGATACATGGTCCTTGGAAAGGAAGCATTGCTGCGCTCCGATGCCCCCCGAACA
  CATGTGACCCACCATGTAAGACCTGAAGGGAATGTCACCCTGAGGTGCTGGGCCCTGGGCTTCTACCCAG
  CTGACATAACCATGACTTGGAAGAGGGATGGGAACAACCACACCCAAGACATGGAGCTGCCAGACACCAG
  GCCTGCAGGGGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTTCCTTCTGGAGAAGAGCTGAGATAC
  ACATGTCATGTGCACCATAAAGGGTTACCTGAGCCTCTCAAGCTGAAATGGGAGCCTCCACACACCATCC
  CCATCATAGCAATTCTCATCGGCCTGGTTCTTGGAACTTTGGTGGTGGGAACTGTGGTCATTTTTCTGGT
  GTGGAAGAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177637
• Insert Size: 993 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177637.3, NP_808305.2
• RefSeq Size: 1403 bp
• RefSeq ORF: 993 bp
• Locus ID: 224761
• Cytogenetics: 17 19.16 cM