Mcat (NM_001030014) Mouse Untagged Clone

CAT#: MC212712

Mcat (untagged) - Mouse malonyl CoA:ACP acyltransferase (mitochondrial) (Mcat), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mcat
• Synonyms: AI225907; BC025519
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212712 representing NM_001030014
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCTCGGGTAGCTCGGGCCGGGTGGGCCTGGCGCTCCTGGGGCCGCCGCGCCGCCTCGAGCCTCC
  GGGAGCCGCCGCCGGACGCCGTGGACGTGGCGGAGCTGCTGCGGGACTCCAGCGTGGCCGAGGAGGGAGC
  CCAGGAGGCGGTGGCGCGGCGGCGGCCACCTTCACAGTGCTCCGTGTTGCTCTTCCCCGGCCAGGGCTGC
  CAGGCGGTCGGCATGGGCAGCGGCCTGCTCCACCTCCCGCGTGTGCGGCAGCTCTACGAGGCCGCGCACC
  GGGTACTGGGCTACGATCTGCTGGAGCTGTGCCTGCGGGGGCCCCAGGAGGACCTGGACCGCACAGTGCA
  TTGCCAGCCCGCCGTCTTTGTGGCTTCACTGGCCGCCGTGGAGAAGCTACATCATCTGCAGCCGGCGGTC
  ATCGACAACTGTGTTGCTGCGGCTGGCTTCAGTGTGGGAGAGTTTGCTGCCTTGGTATTTGCTGGAGCCA
  TGGATTTTTCTGAAGGTCTGTATGCGGTGAAGGCCCGTGCCGAAGCTATGCAAGAAGCATCCGAAGCCGT
  TCCCAGTGGGATGCTGTCTGTCCTTGGCCAGCGTCAGTCCAACTTCTCCTTTGCCTGCTTGGAAGCCCAG
  GAGCACTGCAAGTCTTTGGGTATAGAGAACCCAGTGTGCCAGGTGTCCAACTACCTCTTTCCTGACTGCA
  GGGTCATCTCAGGACACCTTGAGGCCCTTCAGTTTCTCCGGAGGAATTCTGCTAAGTATCACTTCCGGCG
  CACCAAGATGCTGCCTGTAAGTGGAGGCTTCCATACCTGCCTCATGGAGCCAGCCGTGGATCCCCTGATG
  AAAGTTCTAGGCTCGATCAACATCAAGAAGCCACTGGTTGCCGTCCATTCCAATGTCAGTGGGCAGAAGT
  ACACACATCCACAACACATCCGAAAGCTGCTAGGGCAGCAGGTGGTGTCGCCAGTGAAATGGGAACAGAC
  AATGCACTCTATCTACGAGCGGAAGAAAGGCATGGAGTTCCCCAGCACCTACGAGGTGGGCCCCGGGCAG
  CAGCTGGGAAGCATCCTCAAGTGCTGCAACAGGCAGGCGTGGAAGTCCTACAGCCATGTGGATGTGATGC
  AGAACATCATGGATCCTGACCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001030014
• Insert Size: 1146 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030014.2, NP_001025185.1
• RefSeq Size: 1893 bp
• RefSeq ORF: 1146 bp
• Locus ID: 223722
• UniProt ID: Q8R3F5
• Cytogenetics: 15 E1
• Gene Summary: Catalyzes the transfer of a malonyl moiety from malonyl-CoA to the free thiol group of the phosphopantetheine arm of the mitochondrial ACP protein (NDUFAB1). This suggests the existence of the biosynthesis of fatty acids in mitochondria (By similarity).[UniProtKB/Swiss-Prot Function]