Atxn7l3 (NM_001098837) Mouse Untagged Clone

CAT#: MC212664

Atxn7l3 (untagged) - Mouse ataxin 7-like 3 (Atxn7l3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atxn7l3
• Synonyms: E030022H21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212664 representing NM_001098837
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGGAGGAAATGTCTTTGTCTGGCCTGGATAACAGCAAACTAGAGGCCATCGCTCAGGAGATAT
  ATGCTGACCTGGTCGAGGATTCTTGTTTGGGATTCTGCTTTGAAGTACACCGGGCTGTTAAGTGTGGCTA
  CTTCTTCCTGGATGATACGGATCCTGACAGCATGAAGGATTTTGAGATCGTGGACCAGCCTGGGTTGGAC
  ATCTTTGGACAGGTTTTCAACCAGTGGAAGAGCAAGGAGTGTGTTTGCCCCAATTGCAGCCGCAGTATTG
  CTGCTTCCCGCTTTGCCCCCCACCTGGAGAAGTGCCTGGGAATGGGTCGCAACAGCAGCCGAATCGCCAA
  CCGTCGGATTGCCAATAGCAACAATATGAACAAGTCTGAGAGTGACCAAGAAGACAACGATGACATCAAT
  GACAATGACTGGTCCTATGGCTCAGAGAAGAAAGCCAAGAAGAGAAAATCAGACAAGAACCCTAATTCCC
  CTCGAAGATCCAAGTCTCTAAAACACAAAAATGGGGAACTTAGCAACTCCGATCCTTTTAAGTATAGCAA
  CTCAACTGGGATCAGCTATGAGACCCTGGGTCCAGAGGAGCTGCGGAGCCTGCTCACCACGCAATGTGGA
  GTGATTTCTGAACATACCAAGAAGATGTGCACAAGGTCACTACGCTGCCCGCAGCACACGGATGAACAGA
  GGCGAACCGTACGGATTTATTTCCTTGGGCCCTCGGCCGTTCTTCCAGAGGTAGAGAGCTCCTTGGATAA
  CGATGGCTTTGACATGACTGATAGCCAGGCCCTCATCAGCCGGCTTCAGTGGGACGGCTCTTCTGATCTC
  TCACCTTCTGATTCAGGCTCCTCCAAGACTAGTGAAAATCAGGGATGGGGTTTAGGTACCAACAGCTCTG
  AATCACGGAAAACCAAGAAAAAGAAATCCCATCTGAGCTTGGTAGGGACTGCCTCTGGCCTGGGCTCCAA
  CAAGAAGAAAAAGCCAAAGCCACCGGCTCCCCCAACGCCCAGCATCTATGATGACATCAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001098837
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098837.1, NP_001092307.1
• RefSeq Size: 3702 bp
• RefSeq ORF: 1044 bp
• Locus ID: 217218
• UniProt ID: A2AWT3
• Cytogenetics: 11 D
• Gene Summary: Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deubiquitination. Within the SAGA complex, participates in a subcomplex that specifically deubiquitinates both histones H2A and H2B. The SAGA complex is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation. Within the complex, it is required to recruit USP22 and ENY2 into the SAGA complex. Regulates H2B monoubiquitination (H2Bub1) levels. Affects subcellular distribution of ENY2, USP22 and ATXN7L3B.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.