Socs2 (NM_007706) Mouse Untagged Clone

CAT#: MC212637

Socs2 (untagged) - Mouse suppressor of cytokine signaling 2 (Socs2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Socs2
• Synonyms: 8030460M17; AI527257; AW108012; CIS2; Cish2; D130043N08Rik; hg; JAB; SOCS-2; SSI-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212637 representing NM_007706
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCTGCGGTGCCTGGAGCCCTCCGGGAATGGAGCGGACAGGACGCGGAGCCAGTGGGGGACCGCGG
  GGTTGCCGGAGGAACAGTCCCCCGAGGCGGCGCGTCTGGCGAAAGCCCTGCGCGAGCTCAGTCAAACAGG
  ATGGTACTGGGGAAGTATGACTGTTAATGAAGCCAAAGAGAAATTAAAAGAGGCTCCAGAAGGAACTTTC
  TTGATTAGAGATAGTTCGCATTCAGACTACCTACTAACTATATCCGTTAAGACGTCAGCTGGACCGACTA
  ACCTGCGGATTGAGTACCAAGATGGGAAATTCAGATTGGATTCTATCATATGTGTCAAGTCCAAGCTTAA
  ACAGTTTGACAGTGTGGTTCATCTGATTGACTACTATGTCCAGATGTGCAAGGATAAACGGACAGGCCCA
  GAAGCCCCACGGAATGGGACTGTTCACCTGTACCTGACCAAACCTCTGTATACATCAGCACCCACTCTGC
  AGCATTTCTGTCGACTCGCCATTAACAAATGTACCGGTACGATCTGGGGACTGCCTTTACCAACAAGACT
  AAAAGATTACTTGGAAGAATATAAATTCCAGGTATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007706
• Insert Size: 597 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007706.4, NP_031732.1
• RefSeq Size: 2222 bp
• RefSeq ORF: 597 bp
• Locus ID: 216233
• UniProt ID: O35717
• Cytogenetics: 10 49.35 cM
• Gene Summary: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. All four variants encode the same protein.