Astl (NM_172539) Mouse Untagged Clone

CAT#: MC212618

Astl (untagged) - Mouse astacin-like metalloendopeptidase (M12 family) (Astl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Astl
• Synonyms: 7420700F21; C87576; Sas1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212618 representing NM_172539
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCACCCTCAGCATCCAGATGTTCTGGAGTCTGCAGTACCAGTGTTCCAGAAGGCTTCACTCCTG
  AGGGAAGCCCGGTATTTCAGGACAAGGACATCCCCGCAATTAACCAAGGGCTCATCTCAGAGGAGACCCC
  AGAAAGCAGCTTCCTGGTAGAAGGGGACATTATCCGGCCAAGCCCTTTCCGATTGTTGTCAGTGACCAAT
  AATAAATGGCCCAAGGGCGTTGGTGGCTTTGTGGAGATCCCCTTCCTGCTTTCCAGAAAGTATGATGAAC
  TCAGCCGCCGGGTCATTATGGATGCCTTTGCTGAGTTTGAACGTTTCACATGCATCCGGTTTGTTGCCTA
  CCATGGTCAGAGAGACTTTGTTTCCATTCTTCCTATGGCGGGGTGTTTCTCTGGTGTGGGACGCAGTGGA
  GGGATGCAGGTGGTGTCCTTGGCACCCACTTGTCTCCGGAAGGGCCGAGGCATTGTCCTACATGAGCTCA
  TGCACGTACTTGGCTTCTGGCATGAGCATTCACGGGCAGATCGGGACCGCTACATCCAAGTCAACTGGAA
  CGAGATCCTCCCGGGCTTTGAAATCAACTTCATCAAGTCACGGAGTACCAATATGTTAGTTCCCTATGAC
  TACTCATCTGTGATGCATTATGGGAGATTTGCCTTCAGCTGGCGTGGGCAGCCCACCATCATACCACTCT
  GGACCTCCAGTGTTCACATTGGCCAGCGATGGAACCTGAGTACCTCAGATATCACCCGGGTCTGCAGGCT
  GTATAACTGCAGCCGGAGTGTCCCTGACTCCCACGGGAGAGGGTTTGAGGCCCAGAGTGATGGAAGCAGC
  CTCACCCCTGCCTCTATATCACGTCTACAAAGACTTCTCGAGGCACTGTCAGAGGAATCTGGAAGCTCTG
  CCCCTAGTGGCTCCAGGACTGGAGGCCAGAGTATTGCCGGGCTTGGTAACAGCCAGCAAGGATGGGAGCA
  TCCTCCTCAGAGCACATTCAGTGTGGGAGCCTTGGCAAGACCACCTCAGATGCTAGCCGATGCTTCAAAA
  TCGGGGCCTGGAGCAGGTGCAGACAGCTTGTCTCTAGAGCAGTTCCAGCTAGCCCAGGCCCCCACTGTAC
  CTCTTGCTCTATTTCCAGAAGCCAGAGACAAGCCAGCACCTATCCAAGATGCCTTTGAGAGGCTAGCTCC
  ACTTCCAGGAGGCTGTGCACCTGGAAGTCACATTAGAGAGGTGCCCAGAGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172539
• Insert Size: 1245 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172539.3, NP_766127.1
• RefSeq Size: 2392 bp
• RefSeq ORF: 1245 bp
• Locus ID: 215095
• UniProt ID: Q6HA09
• Cytogenetics: 2 F1
• Gene Summary: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.