Vgll2 (NM_153786) Mouse Untagged Clone

CAT#: MC212617

Vgll2 (untagged) - Mouse vestigial like 2 homolog (Drosophila) (Vgll2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vgll2
• Synonyms: C130057C21Rik; Vgl; vgl-2; Vi; VIT; VITO-1; Vito1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212617 representing NM_153786
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGTCTGGATGTTATGTACCAGGTCTACGGTCCCCCGCAGCCTTATTTCGCAGCCGCCTACACTC
  CCTACCACCAGAAACTAGCCTACTACTCAAAAATGCAGGAAGCTCAAGAGTGCGCCAGCCCTGGCAGCAG
  TGCCAGCGGGAGCTCCTCATTTTCCAACCCAACCCCAGCCAGTGTCAAGGAAGAGGAGGGCAGCCCAGAG
  AAAGAGCGCCCGCCCGAAGCTGAGTACATCAACTCCAGATGTGTCCTCTTCACCTACTTCCAGGGGGACA
  TCAGCTCTGTGGTGGACGAACATTTCAGTAGGGCCCTTAGCCACCCAAGCAGCTACACCCCAAGCTGTAC
  CAGCAGCAAAGCACACAGAAGCTCTGGACCCTGGAGAGCAGAAGGCACCTTCCCGATGAGCCAGCGCAGC
  TTCCCCGCCTCCTTCTGGAACAGCGCGTACCAGGCGCCTGTGCCCGCGCCACTAGGCAGTCCTCTGGCCG
  CCGCACACTCGGAGCTGCCCTTTGCCACCGACCCCTACTCTCCCGCCACTCTGCACGGCCACCTGCACCA
  GGGCGCGGCCGACTGGCACCACGCGCACCCGCACCACGCGCACCCGCACCATCCCTATGCGCTGGGCGGC
  GCCCTGGGAGCACAAGCCTCTGCCTACCCGCGGCCAGCAGTGCACGAGGTCTACGCGCCCCACTTCGACC
  CGCGCTATGGGCCGCTGCTCATGCCCGCGGCCACTGGCCGCCCCGGCCGCCTGGCCCCTGCCTCGGCGCC
  GGCTCCCGGCAGCCCTCCCTGCGAGCTTGCGGCCAAGGGCGAGCCGGCGGGCAGCGCATGGGCTGCGCCC
  GGGGGACCCTTCGTGAGCCCCACGGGGGATGTGGCCCAGAGCCTGGGTCTCAGCGTGGACTCAGGTAAGC
  GGAGGAGGGAATGCAGTCTCCCCTCTGCCCCTCCGGCACTGTACCCGACTCTGGGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153786
• Insert Size: 969 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153786.1, NP_722481.1
• RefSeq Size: 1667 bp
• RefSeq ORF: 969 bp
• Locus ID: 215031
• UniProt ID: Q8BGW8
• Cytogenetics: 10 B3
• Gene Summary: This gene is a member of the Vestigial-like (Vgl) gene family and is upregulated during muscle differentiation. The product of this gene interacts with and modifies the DNA-binding properties of the transcription factor, TEF-1, and is important for muscle tissue development. Reduced expression of this gene leads to a reduction in the terminal differentiation of muscle cells. Alternate splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.