Rho (NM_145383) Mouse Untagged Clone

CAT#: MC212573

Rho (untagged) - Mouse rhodopsin (Rho), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rho
• Synonyms: Noerg1; Opn2; Ops; RP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212573 representing NM_145383
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGCACAGAGGGCCCCAATTTTTATGTGCCCTTCTCCAACGTCACAGGCGTGGTGCGGAGCCCCT
  TCGAGCAGCCGCAGTACTACCTGGCGGAACCATGGCAGTTCTCCATGCTGGCAGCGTACATGTTCCTGCT
  CATCGTGCTGGGCTTCCCCATCAACTTCCTCACGCTCTACGTCACCGTACAGCACAAGAAGCTGCGCACA
  CCCCTCAACTACATCCTGCTCAACTTGGCCGTGGCTGACCTCTTCATGGTCTTCGGAGGATTCACCACCA
  CCCTCTACACATCACTCCATGGCTACTTCGTCTTTGGGCCCACAGGCTGTAATCTCGAGGGCTTCTTTGC
  CACACTTGGAGGTGAAATCGCCCTGTGGTCCCTGGTGGTCCTGGCCATTGAGCGCTACGTGGTGGTCTGC
  AAGCCGATGAGCAACTTCCGCTTCGGGGAGAATCACGCTATCATGGGTGTGGTCTTCACCTGGATCATGG
  CGTTGGCCTGTGCTGCTCCCCCACTCGTTGGCTGGTCCAGGTACATCCCTGAGGGCATGCAATGTTCATG
  CGGGATTGACTACTACACACTCAAGCCTGAGGTCAACAACGAATCCTTTGTCATCTACATGTTCGTGGTC
  CACTTCACCATTCCTATGATCGTCATCTTCTTCTGCTATGGGCAGCTGGTCTTCACAGTCAAGGAGGCGG
  CTGCCCAGCAGCAGGAGTCAGCCACCACTCAGAAGGCAGAGAAGGAAGTCACCCGCATGGTTATCATCAT
  GGTCATCTTCTTCCTGATCTGCTGGCTTCCCTACGCCAGTGTGGCCTTCTACATCTTCACCCACCAGGGC
  TCCAACTTCGGCCCCATCTTCATGACTCTGCCAGCTTTCTTTGCTAAGAGCTCTTCCATCTATAACCCGG
  TCATCTACATCATGTTGAACAAGCAGTTCCGGAACTGTATGCTCACCACGCTGTGCTGCGGCAAGAATCC
  ACTGGGAGATGACGACGCCTCTGCCACCGCTTCCAAGACGGAGACCAGCCAGGTGGCTCCAGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145383
• Insert Size: 1047 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC013125, AAH13125
• RefSeq Size: 3249 bp
• RefSeq ORF: 1047 bp
• Locus ID: 212541
• UniProt ID: P15409
• Cytogenetics: 6 53.72 cM
• Gene Summary: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (PubMed:9020854). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:27353443).[UniProtKB/Swiss-Prot Function]