March11 (NM_177597) Mouse Untagged Clone

CAT#: MC212549

41344 (untagged) - Mouse membrane-associated ring finger (C3HC4) 11 (March11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: March11
• Synonyms: 9630025C22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212549 representing NM_177597
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACGAGGGCAAAAAACGGTCTAGCCGGGCGGACAGCCTCGAAGCTGAGCCACCTCTGCCCCCGC
  CGCCCCCACCCCCGCCTCCGGGAGAGTCGGTCCTGGTCCCCACCTCCCCACGCTACCGGCCGCCGCTGCC
  CGCGCCTCTGGAGCGCATCGTAGGGTCGGGCGAGCCGCCGGTAGAACTGGCCCCTCGGCGCAAGGGCGCA
  GGAGAGCCTCTACCGCCGCTGCCGCCGTCTCTGTTGCCCGGCGACCAGGAGGTGACAGCGGCGGGCGACT
  CCTGCGAAGGTCCTAGGCGCCTTCCAGAGGTGAAGCTCCCCGAGGCTGCGACGGGGAAAGGCAGCCCGGG
  GGAGCCCGAGGCCGGCGCGTGCCGGGAGGGCGAGCGGCGAGGCACCGGAGACCAGCCCGAAACACGCTCC
  GTATGTAGCAGCCGCAGCAGCAGTAGTGGCGGCGGTAGCGACCAGCGCTCGGGGCACCAGCACCAGCACC
  ATCAGCCCATTTGCAAGATCTGCTTCCAGGGCGCAGAGCAGGGTGAGTTGTTAAATCCTTGCCGATGTGA
  TGGGTCTGTTCGATACACACATCAGCTGTGTCTTCTAAAGTGGATCAGTGAGAGAGGCTCCTGGACCTGC
  GAGCTGTGCTGTTATAGATACCACGTCACAGCCATTAAAATGAAGCAACCTTGCCAGTGGCAGAGCATTT
  CTATAACACTGGTTGAGAAAGTTCAGATGATCGCTGTAATCCTAGGATCCCTGTTCTTAATAGCGAGTGT
  GACTTGGCTCCTCTGGTCAGCCTTCAGCCCCTATGCAGTGTGGCAGAGGAAGGACATCCTTTTTCAAATC
  TGCTATGGAATGTATGGTTTTATGGATCTAGTGTGCATAGGCCTCATTGTGCATGAAGGAGCTGCCGTTT
  ACAGAGTGTTTAAGCGATGGCGAGCTGTTAATTTGCACTGGGATGTGTTAAATTATGACAAAGCCACAGA
  CATTGAAGAAAGCAGCCGAGGAGAGTCTTCCACAAGTAGGACTTTGTGGCTACCATTGTCAGCTCTGAGG
  AACAGGAACTTGGTCCACCCAACGCAACTAACCTCACCACGGTTTCAGTGTGGCTATGTGCTATTACATC
  TATTCAATCGGATGAGGGCCCATGAAGATGTATCGGAAGATAACGGCTCTGGGGAGGTAGTGATGAGAGT
  GACGTCTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177597
• Insert Size: 1203 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177597.6, NP_808265.2
• RefSeq Size: 1584 bp
• RefSeq ORF: 1203 bp
• Locus ID: 211147
• UniProt ID: Q8CBH7
• Cytogenetics: 15 B1
• Gene Summary: E3 ubiquitin-protein ligase that mediates polyubiquitination of CD4. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May play a role in ubuquitin-dependent protein sorting in developmenting spermatids.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).