Pgbd5 (NM_171824) Mouse Untagged Clone

CAT#: MC212533

Pgbd5 (untagged) - Mouse piggyBac transposable element derived 5 (Pgbd5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pgbd5
• Synonyms: 2900019M05Rik; AI854313
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212533 representing NM_171824
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCCAGCGCTAGCGCGGTGGACTTCTTCCAGCTCTTCGTCCCGGACAACGTGCTCAAGAACATGG
  TGGTACAGACCAACATGTATGCCAGGAAGTTCCAGGAGCGGTTTGGCAGTGACGGTGCCTGGGTGGAGGT
  GACGTTGGCAGAGATGAAGGCCTTCCTGGGCTATGTGATCTCCACCAGCGTCTCGCACTGTGAGTCGGTA
  CTCAGCATTTGGAGCGGAGGCTTCTATAGCAACCGGAGCCTTGCCCTCGTCATGAGCCAGGCCCGCTTCG
  AGAAAATCCTCAAGTACTTCCATGTTGTGGCCTTCCGATCCAGCCAGACCACGCATGGGCTCTACAAGGT
  CCAGCCCTTCCTCGACTCCCTGCAGAGTGGCTTTGATGCTGCTTTCAGGCCGTCTCAGACCCAGGTGCTA
  CATGAACCCCTGATTGACGAGGACCCTGTGTTCATTGCCACGTGCACGGAGCGAGAGCTGCGGAAGAGGA
  AAAAGCGGAAATTCAGCCTGTGGGTCCGCCAGTGCTCTTCAACCGGGTTCATCATCCAGATCTACGTCCA
  CCTAAAGGAAGGTGGTGGCCCAGATGGCCTGGACGCTCTGAAGAACAAGCCACAGCTGCACAGCATGGTG
  GCCCGGAGCCTGTGCCGGAACGCGGCCGGAAAGAACTATATCATCTTTACAGGGCCCAGCATCACCAGCC
  TCAATCTGTTCGAAGAATTTGAGAAGCAAGGGATCTACTGCTGTGGCCTCCTCAGCTCTAGGAAGAGTGA
  CTGCACAGGTCTCCCTCCATCCATGTTGACCAACCCTGCCACCCCACTCGCCCGGGGCCAGCACCAGATC
  AGGACAAAGGGCAACATGTCACTGATCTGCTGGTACAACAAGGGGCACTTCCGCTTCCTGACCAACGCCT
  ACTCCCCTGTGCAGAAAGGCGTCATCATCAAGAGGAGGAGTGGGGAAATCCCCTGCCCTCTGGCCGTGGA
  GGCCTTTGCGGCTCACCTCAGCTACATCTGCAGATATGACGATAAGTACAGCAAGTATTTCATCTCTCAC
  AAGCCAAACAAGACGTGGCAGCAAGTGTTCTGGTTCGCCATCAGCATCGCCGTCAACAACGCCTACATCC
  TGTACAAAATGTCAGACGCCTACCACGTGAAGAAGTACAGCCGGGCACAGTTCGGAGAGAGACTTGTCAG
  GGAGTTGCTGGGCTTGGAGGACTCATCGCCAGCCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_171824
• Insert Size: 1230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_171824.2, NP_741958.1
• RefSeq Size: 2854 bp
• RefSeq ORF: 1230 bp
• Locus ID: 209966
• UniProt ID: D3YZI9
• Cytogenetics: 8 E2
• Gene Summary: The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]