Btla (NM_177584) Mouse Untagged Clone

CAT#: MC212498

Btla (untagged) - Mouse B and T lymphocyte associated (Btla), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Btla
• Synonyms: A630002H24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212498 representing NM_177584
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACAGTGCCTGCCATGCTTGGGACTCCTCGGTTATTTAGGGAATTCTTCATCCTCCATCTGGGCC
  TCTGGAGCATCCTTTGTGAGAAAGCTACTAAGAGGAATGATGAAGAGTGTCCAGTGCAACTTACTATTAC
  GAGGAATTCCAAACAGTCTGCCAGGACAGGAGAGTTATTTAAAATTCAATGTCCTGTGAAATACTGTGTT
  CATAGACCTAATGTGACTTGGTGTAAGCACAATGGAACAATCTGTGTACCCCTTGAGGTTAGCCCTCAGC
  TATACACTAGTTGGGAAGAAAATCAATCAGTTCCGGTTTTTGTTCTCCACTTTAAACCAATACATCTCAG
  TGATAATGGGTCGTATAGCTGTTCTACAAACTTCAATTCTCAAGTTATTAATAGCCATTCAGTAACCATC
  CATGTGACAGAAAGGACTCAAAACTCTTCAGAACACCCACTAATAATATCTGACATCCCAGATGCCACCA
  ATGCCTCAGGACCATCCACCATGGAAGAGAGGCCAGGCAGGACTTGGCTGCTTTACACCTTGCTTCCTTT
  GGGGGCATTGCTTCTGCTCCTTGCCTGTGTCTGCCTGCTCTGCTTTCTGAAAAGGATCCAAGGGAAAGAA
  AAGAAGCCTTCTGACTTGGCAGGAAGGGACACTAACCTGGTTGATATTCCAGCCAGTTCCAGGACAAATC
  ACCAAGCACTGCCATCAGGAACTGGAATTTATGATAATGATCCCTGGTCTAGCATGCAGGATGAATCTGA
  ATTGACAATTAGCTTGCAATCAGAGAGAAACAACCAGGGCATTGTTTATGCTTCTTTGAACCATTGTGTT
  ATTGGAAGGAATCCAAGACAGGAAAACAACATGCAGGAGGCACCCACAGAATATGCATCCATTTGTGTGA
  GAAGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177584
• Insert Size: 918 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177584.3, NP_808252.1
• RefSeq Size: 3232 bp
• RefSeq ORF: 918 bp
• Locus ID: 208154
• UniProt ID: Q7TSA3
• Cytogenetics: 16 B5
• Gene Summary: Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2).