Eme2 (NM_001163102) Mouse Untagged Clone

CAT#: MC212455

Eme2 (untagged) - Mouse essential meiotic endonuclease 1 homolog 2 (S. pombe) (Eme2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eme2
• Synonyms: 2810013J18Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212455 representing NM_001163102
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGTTGGTCCCGGGAGAGTCACGGTCTCGCGTCTCGGTCGGGGCCTGCGTCTCGGCCATCGGC
  GACCTCAAACCTGGGAGATTTCCGACTCAGACGGTGAAGGTGTCCCCGCTAGGGAGGTCGGCACGCAAGC
  CCCGAGTCCAGCAGGAGAGCGCAGAGCTGCAGCCAAGGCTTTACGGGCAGATCAGGTTCTGGGGCGACTG
  GTGGTGTGCGTGGACCCAGCTGTCCTGGAAGATGCAGGCTCTGACATCCTGATGGAGGCGCTGGGCACGC
  TAGGCTGTGAATGCCGCATCGAACCGCAGCACCAGGCCCGCAGCCTGCAGTGGAACGTTGTCAGGCCCGA
  CCCGGCCCCCAGCAATGTGCCTCTGGAGGCGAAGGCTGAAAATGAGCAGGAACAGTTGCTGCTGCTGGAA
  CCCCAGGAATTTCTTCAGGGTGCTGCGCAGCTGACCCAGATCACAGATCCACCTTGCTCCATCCCCTGGC
  TTTCTCCCAAGAGTCTCACCCGCTCCCATCTGGCTGTCATTGGACTGGACGCATACCTGTGGTCTCACCA
  GCTCAGTTCTCAGAAGACATGGCAACTAAAGAAGTCAAAGGAAGCCCATGCCAGGGGAGCCATCAGCTGG
  GCTGAGGTGGAGGAGATCCTGGTGCTGCTGCAGCTCCATGCAAACCTGGATGTGCTGCTGATGGCTTCGT
  GGCAGGAGCTGAGTCAGTACGTGTGTGCCTTCACCAGGGCCCTCTCGCAGCTCCCCTCGAAGCAGCACCG
  GGACTCCCAGGCCTTTTCTTTCTGCACAGCAGGGCACTGGGCCTCAGGCCAGCAAGTGACCAGAGATGGC
  TCTGGGCTCAGAGGAGTGTGGTGGCGACAAATCAGACAGTTCAACCGGGTCAGCCCAGCTGTGGCTGACG
  CTGTTGTTACTGCCTTCCCATCACCCCGCCTTTTGCAACAGGCTCTCCTGGACTGCAGCACAGAGCAAGA
  ACGCCTGAGCCTCCTAGCTGATCTCCCTGTGAAGGCCCACAAAGGCAAGCAGCCTCGCAGAGTGGGGCCT
  GACCTCTCACGCAGAATCTGTATCTTCTTGACAACCACCAACCCTGACCTCCTGCTGGACCTGAGCTCTT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163102
• Insert Size: 1122 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163102.1, NP_001156574.1
• RefSeq Size: 1521 bp
• RefSeq ORF: 1122 bp
• Locus ID: 193838
• UniProt ID: Q56A04
• Cytogenetics: 17 A3.3
• Gene Summary: Interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures.[UniProtKB/Swiss-Prot Function]