Syt12 (NM_134164) Mouse Untagged Clone

CAT#: MC212349

Syt12 (untagged) - Mouse synaptotagmin XII (Syt12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syt12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212349 representing NM_134164
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGGACGTGACAGAATATCATCTGAGCGTCATCAAGAGCCCCCCAGGCTGGGAGGTGGGTGTCT
  ATGCTGCCGGGGCCCTGGCTCTGCTGGGAATTGCTGCTGTGAGTCTATGGAAGCTCTGGACATCGGGGAG
  CTTCCCCAGCCCCTCCCCGTTCCCTAATTACGACTACAGGTACCTGCAGCAGAAATATGGAGAGGCCTAT
  GTGGAGGCTAAGCTGAAGCGGGTCCCTCCTTGGAATGACCAGCGGACCACTACTCGGGGACCACCCAGCC
  GCAAAGGCAGCCTTAGTATAGAAGACACCTTTGAAAGCATCAGTGAGCTGGGACCCCTGGAGCTGATGGG
  CCGGGAACTGGACTTAGCCCCCTATGGTACTCTCCGAAAGTCACAGTCGGCTGACTCTCTGAATTCCATC
  TCCTCTGTGAGCAACACCTTTGGGCAGGATTTCACCCTGGGCCAGGTGGAGGTGAGCATGGACTATGATG
  GAGCCTCCCACACCCTCCACGTGGCAGTACTACAAGGCAAAGACCTCCTGGAGCGCGAGGAGGCCACCTT
  CGAGTCCTGCTTCATGCGTGTCAGTCTGCTGCCAGACGAACAGATCGTGGGCATCTCCCGGATCCAGAGG
  AATGCCTATTCCATCTTCTTCGATGAGAAGTTCTCTGTCCCCCTGGATCCCACAGCCCTGGAGGAGAAGA
  GCCTTAGATTTTCTGTGTTTGGTATCGACGAGGATGAGCGGAATGTGAGCACCGGCGTGGTGGAGCTGAA
  GCTGTCCGTTCTGGATCTCCCGCTGCAGCCCTTCAGTGGCTGGCTATACTTGCAGGACCAGAATAAGGCT
  GCTGACGCTGTGGGAGAGATCCTGCTGTCCCTCAGTTACCTGCCCACAGCTGAGCGGCTGACTGTGGTTG
  TGGTGAAAGCCAAGAATCTCATCTGGACCAATGAGAAGAGCACAGCAGACCCCTTCGTCAAAGTATACCT
  GCTGCAGGATGGGAGGAAAATGAGCAAGAAGAAGACGGCTGTGAAGAGGGATGACCCCAACCCAGTGTTC
  AACGAAGCCATGATCTTCTCAGTGCCAGCCATTGTGCTACAGGACCTGTCTCTCCGGGTGACAGTGGCTG
  AGAGCAGCAGCGACGGCCGTGGGGACAACGTGGGCCATGTCATCATTGGGCCAGGGGTCAGTGGCATGGG
  CACCACACATTGGAACCAGATGCTGGCCACACTACGCAGGCCTGTGTCCATGTGGCATCCAGTCCGGCGA
  AACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134164
• Insert Size: 1266 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134164.5, NP_598925.1
• RefSeq Size: 3277 bp
• RefSeq ORF: 1266 bp
• Locus ID: 171180
• UniProt ID: Q920N7
• Cytogenetics: 19 A
• Gene Summary: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.[UniProtKB/Swiss-Prot Function]