Bbc3 (NM_133234) Mouse Untagged Clone

CAT#: MC212335

Bbc3 (untagged) - Mouse BCL2 binding component 3 (Bbc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bbc3
• Synonyms: PUMA; PUMA/JFY1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212335 representing NM_133234
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCGCACGCCAGGAGGGCAGCTCTCCGGAGCCCGTAGAGGGTCTAGCCCGCGACAGTCCGCGCC
  CCTTCCCGCTCGGCCGCCTGATGCCCTCCGCTGTATCCTGCAGCCTTTGCGAGCCCGGCCTGCCCGCCGC
  CCCTGCTGCCCCTGCCTTGCTGCCGGCCGCCTACCTCTGCGCCCCCACCGCTCCACCTGCCGTCACCGCC
  GCCCTGGGGGGCCCCCGCTGGCCTGGGGGTCACCGCAGCCGGCCCAGAGGCCCGCGCCCGGACGGTCCTC
  AGCCCTCCCTGTCACCAGCCCAGCAGCACTTAGAGTCGCCCGTGCCCAGCGCCCCGGAGGCCCTGGCAGG
  AGGCCCCACCCAAGCTGCCCCGGGAGTGCGTGTGGAGGAGGAGGAGTGGGCCCGGGAGATCGGGGCCCAG
  CTGCGGCGGATGGCGGACGACCTCAACGCGCAGTACGAGCGGCGGAGACAAGAAGAGCAGCATCGACACC
  GACCCTCACCCTGGAGGGTCATGTACAATCTCTTCATGGGACTCCTCCCCTTACCCAGGGATCCTGGAGC
  CCCAGAAATGGAGCCCAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133234
• Insert Size: 582 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133234.2, NP_573497.1
• RefSeq Size: 1976 bp
• RefSeq ORF: 582 bp
• Locus ID: 170770
• UniProt ID: Q99ML1
• Cytogenetics: 7 A2
• Gene Summary: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53 (By similarity). Regulates ER stress-induced neuronal apoptosis.[UniProtKB/Swiss-Prot Function]