Atxn3 (NM_029705) Mouse Untagged Clone

CAT#: MC212229

Atxn3 (untagged) - Mouse ataxin 3 (Atxn3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atxn3
• Synonyms: 2210008M02Rik; AI463012; AI647473; ataxin-3; ATX3; Mjd; MJD1; Sca3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212229 representing NM_029705
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCATCTTCCACGAGAAACAAGAAGGCTCACTTTGTGCTCAGCATTGCCTGAATAACCTATTGC
  AAGGAGAGTATTTTAGCCCTGTGGAGCTATCCTCAATTGCACACCAGCTGGATGAAGAGGAGAGGCTGAG
  AATGGCAGAAGGGGGAGTCACTAGTGAAGACTACCGCACATTTTTACAGCAGCCTTCTGGAAATATGGAT
  GACAGCGGCTTTTTCTCTATTCAAGTTATAAGCAATGCTTTGAAAGTTTGGGGTTTAGAACTAATCCTGT
  TCAACAGTCCAGAGTACCAGAGGCTCAGAATTGATCCTATAAACGAAAGATCCTTTATATGCAATTATAA
  AGAACACTGGTTTACAGTTAGAAAATTAGGCAAGCAGTGGTTTAACTTGAATTCTCTGTTGACGGGTCCA
  GAATTAATATCAGATACATACCTCGCACTATTCTTGGCTCAATTACAGCAAGAAGGTTATTCTATATTTG
  TTGTTAAGGGTGATCTGCCAGATTGTGAAGCTGACCAACTTTTGCAGATGATCAAGGTCCAACAGATGCA
  TCGACCAAAACTTATTGGAGAGGAACTTGCACATCTGAAAGAGCAGAGTGCCCTCAAAGCAGACCTGGAG
  CGCGTCTTAGAAGCAGCTGATGGGTCGGGCATATTTGATGAAGATGAGGATGATTTACAGAGGGCTCTAG
  CCATAAGTCGCCAGGAAATCGACATGGAGGATGAAGAAGCTGATCTCCGCAGGGCCATTCAGCTCAGTAT
  GCAAGGTAGTTCCAGAAGTATGTGTGAAAATAGTCCACAGACATCAAGTCCAGATCTCTCTTCAGAAGAG
  CTGCGGAGGAGACGAGAAGCCTACTTTGAAAAGCAACAGCAGCAGCAGCAGGAGGTAGACCGACCTGGAC
  CCCTTTCATATCCACGTGAAAGACCGACCACAAGTTCAGGAGGACGTAGGAGCGACCAAGGAGGCGACGC
  TGTGAGTGAAGAGGACATGCTTCGGGCAGCTGTGACCATGTCTTTAGAAACTGCTAAAGACAACTTGAAA
  GCAGAAAGAAAAAAATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029705
• Insert Size: 1068 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029705.3, NP_083981.2
• RefSeq Size: 5376 bp
• RefSeq ORF: 1068 bp
• Locus ID: 110616
• UniProt ID: Q9CVD2
• Cytogenetics: 12 E
• Gene Summary: Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates (By similarity). Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins (By similarity). Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (PubMed:21855799). Interacts with key regulators of transcription and represses transcription: acts as a histone-binding protein that regulates transcription (By similarity). Regulates autophagy via the deubiquitination of 'Lys-402' of BECN1 leading to the stabilization of BECN1 (PubMed:28445460).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).