Akr7a5 (NM_025337) Mouse Untagged Clone

CAT#: MC212223

Akr7a5 (untagged) - Mouse aldo-keto reductase family 7, member A5 (aflatoxin aldehyde reductase) (Akr7a5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Akr7a5
• Synonyms: 0610025K21Rik; Afar; Afar1; Akr7a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212223 representing NM_025337
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGTGCAGCGTCCAGAGCCGTGGGCCGCGCTGCTGTTCGCTCCGCGCAGCGCTCCGGGACTTCGG
  TCGGGCGTCCTCTCGCCATGTCCCGGCCTCCGCCACCCCGCGCCGCCTCTGGCGCCCCTCTCCGGCCCGC
  TACGGTGCTGGGCACCATGGAGATGGGGCGTCGCATGGATGCGAGTGCTAGCGCCGCTTCGGTCCGCGCC
  TTCCTGGAGCGCGGCCACAGCGAGCTGGACACGGCCTTCATGTACTGCGACGGCCAGTCCGAGAACATCC
  TGGGCGGCCTGGGGCTCGGGCTGGGCAGCGGCGACTGCACAGTGAAAATCGCCACCAAGGCCAACCCCTG
  GGAAGGGAAGTCACTGAAGCCTGACAGCATCCGGTCCCAGTTAGAGACGTCTCTGAAGCGGCTGCAGTGT
  CCCCGGGTGGATCTCTTCTACTTACACGCTCCCGACCACAGCACTCCTGTGGAGGAGACACTTCGTGCCT
  GCCACCAGCTGCATCAGGAGGGCAAGTTTGTGGAGCTTGGTCTGTCTAACTATGCCTCCTGGGAAGTGGC
  TGAGATCTGTACCCTCTGCAAGAGCAACGGCTGGATCCTGCCAACTGTGTACCAGGGCATGTACAACGCC
  ACCACCCGGCAGGTGGAGGCCGAGCTCCTCCCCTGCCTCAGACACTTCGGACTGAGGTTCTACGCCTACA
  ACCCTTTGGCTGGGGGCCTGCTGACTGGCAAGTATAAATACGAAGACAAGGATGGGAAACAACCCGTGGG
  CCGCTTCTTTGGGAATAACTGGGCTGAGACCTACAGGAACCGCTTCTGGAAGGAACACCACTTTGAGGCC
  ATCGCCCTGGTAGAAAAGGCCCTGCAGACCACCTATGGCACCAACGCCCCCCGCATGACCTCGGCCGCCC
  TGCGCTGGATGTACCACCACTCGCAGCTCCAGGGCACCCGAGGGGACGCAGTCATCTTGGGCATGTCCAG
  TCTGGAGCAGCTGGAGCAGAACTTGGCCGCCACTGAGGAAGGGCCCCTGGAGCCGGCTGTCGTGGAAGCC
  TTTGACCAAGCCTGGAACATGGTCGCCCACGAGTGTCCCAACTACTTCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025337
• Insert Size: 1104 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025337.3, NP_079613.3
• RefSeq Size: 1648 bp
• RefSeq ORF: 1104 bp
• Locus ID: 110198
• UniProt ID: Q8CG76
• Cytogenetics: 4 70.64 cM
• Gene Summary: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).[UniProtKB/Swiss-Prot Function]