Tmem74b (NM_001160363) Mouse Untagged Clone

CAT#: MC212183

Tmem74b (untagged) - Mouse RIKEN cDNA 5430405G05 gene (5430405G05Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem74b
• Synonyms: 5430405G05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212183 representing NM_001160363
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTCCCCCTGGTCTGGAACTGAAGACATTGAGCAATGGTCCCCAGGTTCCAAGGAGAGCGGCCT
  CGCAGGGCCCCGTGGCGCCACCCAGGATGGGTGTGGAGAATGCCTGCTTCTTCTCTGAGGAGCAGGAGAC
  TCATTTCCAGAACCCTGGGGATGCCAGACTGGGTAACGCCCCCAGTCCCCCTGGAGGTGTCCCCTCACTG
  TCCCGGTCCCAGCGGGATGATCTTTCTTTGCATTCAGAGGAGGGACCAGGCCTGGAACCTGTGAGCCGCC
  CGGTGGACTATGGCTTTGTTTCTGCTCTGGTTTTCTTGGTGAGCGGAATCCTCCTGGTGGTGACAGCATA
  CGCCATCCCCCGAGAGGCCCGGGTCAACCCTGACACAGTGACAGCAAGGGAGATGGAACGCCTGGAGATG
  TACTACGCCCGCTTGGGCTCACATCTGGACAAATGCATCATTGCGGGCCTCGGGCTGCTCACAGTGGGTG
  GCATGCTCCTCTCTGTGCTGCTCATGGTCTCTCTGTGCAAGGGAGAGCTGTACCGCAGACAGACCTTTGT
  CCCCGGCAGGGGATCCAGAAAGACCTATGGCTCCATCAACCTGCGTATGAGGCAGCTCACTGGGGACGGA
  GGCCAGGTCCTAGTAGAGAATGAGGTTGTTCAAGTCTCAGAGACCAGCTATACCACTCAGGGCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001160363
• Insert Size: 699 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160363.1, NP_001153835.1
• RefSeq Size: 1448 bp
• RefSeq ORF: 699 bp
• Locus ID: 108832
• Cytogenetics: 2 G3