B3gnt5 (NM_001159407) Mouse Untagged Clone

CAT#: MC212171

B3gnt5 (untagged) - Mouse UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 (B3gnt5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: B3gnt5
• Synonyms: beta3GnT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212171 representing NM_001159407
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTTGTTAGCAGAAGAGTCAAAAGATGGAAAATTTTTCACTTTTTTGTCACTTGTTTTATAT
  TAAGCTTCATGGTTTTTTGGAGCCCAATCAATAATTACATCATGAGCCATATGAAGTCCTACTCCTACAG
  ATACCTCGTAAATAGCTATGGCTTTGTAAACAATTCCCTGTCTCTCAAGCACAGCTCTGTGCAGCCTCAC
  TACCCATACTTGATCAACCACAGAGAGAAGTGTCAGGCTCAAGATGTCCTCCTCTTACTGTTTATAAAGA
  CTGCCCCTGAAAACTATGGCCGACGTTCTGCAATCAGAAAAACGTGGGGCAATGAGAACTATGTTCAGTC
  TCAACTCAATGCCAACATCAAAATTCTGTTTGCATTAGGAACTCCTGGTCCACTGAAGGGAAAAGAACTG
  CAAAAAAGACTAATCGGGGAAGATCAAGTGTACAAGGATATAATTCAGCAAGATTTCATTGATTCTTTCC
  ACAATCTTACTTCTAAATTCCTTCTTCAGTTCAGCTGGGCAAATACCTTTTGTCCACATGCCAAATTCCT
  GATGACTGCTGATGATGATATATTTATCCACATGCCAAATCTCATTGAATATCTTCAAGGGCTAGAGCAG
  ATTGGAGTTCGAGACTTTTGGATTGGTCACGTTCATCGAGGTGGCCCTCCTGTTAGGGATAAAAGCAGCA
  AATACTATGTTCCCTATGAAATGTACAAGTGGCCAGCCTACCCTGACTATACAGCTGGTGCTGCCTATGT
  TGTCTCCAGAGATGTAGCTGCCAAAATCTATGAGGCATCGCAGACACTGAATTCCAGTATGTACATAGAT
  GATGTATTCATGGGCCTCTGTGCCAATAAAGTGGGAATCTTGCCACAGGACCATGTATTTTTCTCTGGGG
  AAGGGAAAATTCCTTATCACCCCTGCATCTATGAAAAGATGATGACATCTCACGGACACTTACAAGATCT
  GCAGGACCTCTGGATAGAGGCTACACATCCTAAAGTAAAGAACATTTCAAAAGGGTTTTTTGGTCAAATA
  TACTGCAGGTTAATTAAGATAGTTCTTCTCTGCAGACTGACTTACAGGAATTCATACCCTTGTTGGGCTG
  CATTTGCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159407
• Insert Size: 1131 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159407.1, NP_001152879.1
• RefSeq Size: 4872 bp
• RefSeq ORF: 1131 bp
• Locus ID: 108105
• UniProt ID: Q8BGY6
• Cytogenetics: 16 A3
• Gene Summary: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.