Bmt2 (NM_175312) Mouse Untagged Clone

CAT#: MC212068

B630005N14Rik (untagged) - Mouse RIKEN cDNA B630005N14 gene (B630005N14Rik), (10ug)


  "NM_175312" in other vectors (4)

Reconstitution Protocol

USD 503.00

5 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Bmt2"

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Bmt2
Synonyms AI666701; B630005N14Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC212068 representing NM_175312
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGGGGCCGGGTGGCCGAGGCGCAGCGCGCGGACAGCGGCCCCCGAACGCCGCGCAGCCCCGGG
AGCAGGAGCGGAAGCTGGAGCAGGAGAAGCTGTCCGGGGTGGTGAAGAGCGTTCATCGGCGGCTCCGCAA
GAAGTACCGCGAAGTGGGAGACTTTGATAAGATCTGGCGAGAACACTGTGAAGATGCGGAAACACTTTGC
GAATATGCTGTTGCAATGAAAAATCTGGCAGATAATCACTGGGCAAAAACTTGTGAGGGTGAAGGTCGCA
TTGAATGGTGTTGTAGTGTATGCAGAGAGTACTTCCAAAATGGTGGGAAAAGAAAAGCACTTGAGAAAGA
TGAAAAACGAGCTGTCCTCGCCACTAAGACCACTCCAGCCTTAAACGTGCACGAGTCTTCTAAACTTGAA
GGTCCTTTAACAAACCTCAGCTTTACAAGCCCTGACTTTATAACTGAGTTGCTTCAAGCCTCAGGAAAGA
TCAGATTACTTGATGTTGGCAGCTGCTTTAATCCATTTTTGAAGTTTGAAGAATTTTTAACTGTTGGCAT
AGATATTGTACCTGCTGTAGAGAGTGTGTATAAGTGTGACTTCCTGAACCTGCAGCTGCAGCAGCCACTC
CAGCTTGCACAAGATGCTATAGATGCTTTTCTGAAGCAGCTGAGAAATCCTATTGATGCTCTTCCGGGGG
AACTTTTTCATGTGGTTGTCTTCTCTCTCCTTCTTTCCTATTTTCCATCACCCTACCAGCGATGGATTTG
CTGCAAGAAAGCCCATGAGCTGTTAGTCCTGAACGGCTTATTGCTCATCATCACTCCCGATTCCTCCCAT
CAGAACCGGCACGCTATGATGATGAAGAGCTGGAAGATTGCTATAGAATCCCTGGGTTTTAAGCGCTTCA
AGTATTCAAAATTTTCTCATATGCATCTCATGGCTTTTAGAAAAACCTCCCTTAAAACCACAAGTGACTT
GGTTAGCAGGAACTATCCAGGGATGTTGTATATTCCTCAAGATTTCAACAGTGTAGAAGAGGAGGAGTAT
TCTAACACTTCCTGCTATGTCCGATCAGATCTAGAAGATGAACAACTCGCATACGGTTTCACGGAGCTCC
CCGAAGCTCCTTATGATTCCGATTCTGGAGAAAGCCAAGCCAGCTCTATTCCATTTTATGAGTTAGAGGA
TCCCATCTTACTTTTAAGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175312
Insert Size 1212 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175312.4, NP_780521.2
RefSeq Size 4147 bp
RefSeq ORF 1212 bp
Locus ID 101148
UniProt ID Q8BXK4
Cytogenetics 6 A1
Gene Summary S-adenosyl-L-methionine-binding protein that acts as an inhibitor of mTORC1 signaling via interaction with the GATOR1 and KICSTOR complexes. Acts as a sensor of S-adenosyl-L-methionine to signal methionine sufficiency to mTORC1: in presence of methionine, binds S-adenosyl-L-methionine, leading to disrupt interaction with the GATOR1 and KICSTOR complexes and promote mTORC1 signaling. Upon methionine starvation, S-adenosyl-L-methionine levels are reduced, thereby promoting the association with GATOR1 and KICSTOR, leading to inhibit mTORC1 signaling. Probably also acts as a S-adenosyl-L-methionine-dependent methyltransferase.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.