4933434E20Rik (NM_027500) Mouse Untagged Clone

CAT#: MC212044

4933434E20Rik (untagged) - Mouse RIKEN cDNA 4933434E20 gene (4933434E20Rik), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4933434E20Rik
• Synonyms: 5730552F22Rik; AI462154; NICE-3; NS5ATP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212044 representing NM_027500
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTAGTAGTAACTGGCTTTCTGGAGTGAATGTCGTGCTGGTGATGGCGTACGGGAGCCTGGTAT
  TCGTACTGCTGTTTATTTTTGTGAAGAGACAAATCATGCGCTTTGCAATGAAATCTAGAAGGGGACCTCA
  TGTTCCTGTAGGACACAATGCCCCCAAGGACTTAAAAGAGGAGATCGATATTCGACTATCCAGGGTTCAA
  GATATCAAGTATGAACCCCAGCTCCTTGCAGATGATGATACCAGACTACTGCAGCTGGAAACCCAGGGGA
  ATCAAAGTTGCTACAACTATCTGTACAGAATGAAAGCTCTAGATGCCATCCGTGCCTCTGAGATCCCGTT
  TCATGCTGAAGGCCGGCACCCCTGTTCTTTAATGGGTAAGAATTTCCGCTCCTACTTGCTAGATCTGCGA
  AATACTAGCACTCCTTTCAAGGGTGTTGGCAAGGCCCTCATTGATACTCTCCTGGATGGATATGAGACAG
  CCCGCTATGGGACAGGGGTCTTTGGCCAGAGTGAGTACTTACGATATCAAGAGGCCTTGAGTGAGCTGGC
  CACTGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027500
• Insert Size: 570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027500.4, NP_081776.2
• RefSeq Size: 885 bp
• RefSeq ORF: 570 bp
• Locus ID: 99650
• UniProt ID: Q8R092
• Cytogenetics: 3 F1
• Gene Summary: General regulator of phagocytosis. Required to uptake Gram negative bacterium by macrophages.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site that is used in variant 1. This results in a frameshift compared to variant 1. The encoded protein (isoform 2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.