Nucks1 (NM_175294) Mouse Untagged Clone

CAT#: MC212035

Nucks1 (untagged) - Mouse nuclear casein kinase and cyclin-dependent kinase substrate 1 (Nucks1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nucks1
• Synonyms: 2700010L10Rik; 8430423A01Rik; AI647518; C78391; Nucks
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212035 representing NM_175294
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGCCTGTCAGAAATAGGAAGGTTGTGGATTACTCTCAGTTTCAGGAATCAGATGATGCAGATG
  AAGATTATGGAAGAGATTCAGGCCCTCCTGCTAAGAAGATTCGGTCATCTCCCCGAGAGGCTAAAAATAA
  GAGGCGATCTGGAAAAAATTCACAAGAAGACAGTGAAGACTCAGAAGAAAAAGATGTGAAGACCAAGAAG
  GATGATTCTCACTCGGCAGAAGACAGCGAAGATGAAAAAGATGATCATAAAAATGTACGCCAGCAACGAC
  AGGCAGCATCTAAAGCAGCTTCTAAGCAGAGAGAGATGCTCTTGGAAGATGTTGGCAGCGAGGAAGAGCC
  GGAAGAAGATGATGAGGCGCCATTCCAGGAGAAAGATTCTGGCAGCGATGAAGATTTCCTAATGGAAGAC
  GACGATGATAGTGATTATGGCAGTTCAAAAAAGAAAAACAAAAAGATGGTTAAGAAGTCCAAACCTGAGA
  GAAAAGAAAAGAAAATGCCCAAACCCAGACTAAAGGCAACAGTGACGCCAAGTCCAGTGAAAGGCAAAGC
  GAAAGTGGGTCGCCCCACAGCTTCGAAGAAATCAAAGGAAAAAACTCCTTCTCCCAAAGAAGAGGATGAG
  GAAGCAGAAAGCCCTCCAGAAAAGAAGTCCGGAGACGAAGGGTCCGAGGATGAAGCCTCATCTGGGGAAG
  ATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175294
• Insert Size: 705 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175294.3, NP_780503.2
• RefSeq Size: 6096 bp
• RefSeq ORF: 705 bp
• Locus ID: 98415
• UniProt ID: Q80XU3
• Cytogenetics: 1 E4
• Gene Summary: Chromatin-associated protein involved in DNA repair by promoting homologous recombination (HR). Binds double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures, but with less affinity than RAD51AP1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.