Prg4 (NM_001110146) Mouse Untagged Clone

CAT#: MC212019

Prg4 (untagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prg4
• Synonyms: CACP; DOL54; JCAP; lubricin; MSF; SZP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212019 representing NM_001110146
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGAAAATACTTCCCGTCTGCTTGTCGCTGCTGCTTCCTGTTGTCTTGATTCAACAGGTTTCAT
  CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTACTCTAGAGATGCCACCTGCAACTGTGA
  TTATAACTGTCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC
  AAAGGACGCTGCTTCGAGTCCTTCGCTCGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTATG
  GCAAGTGCTGTGCAGATTATGACAGCTTCTGTGAAGAAGTAAAAGATAACAAGAAAAACACTCCTAAAAA
  GAAACCCAATCCAGAACCGCCGGCTGTGGATGAAGCTGGAAGCGGGCTGGATAATGCTATTCCAGGCACT
  GATCTTTTGGCCGGGAGACTCAATCAAGGCATTAACATCAATCCCATGCTTTCAGATGAGACCAATTTAT
  GCAATGGTAAGCCAGTGGATGGACTGACTACGCTGCGCAATGGGACATTAGTTGCATTTCGAGGTCATTA
  TTTCTGGATGCTGAATCCATTCAGACCACCATCTCCACCACGCAGAATTACCGAAGTTTGGGGTATTCCC
  TCTCCTATTGACACAGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTTTAAGGATTCTCAGT
  ACTGGCGCTTCACCAATGATGTGGTAGATCCTGGGTATCCTAAACAAATTGTCAAAGGCTTTGGAGGACT
  AACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGGCCTGAATCTGTGTACTTCTTC
  AAGAGAGGTGGCAACATTCAGCAGTACACTTATAAACAGGAGCCAATGAAGAAGTGCACAGGGAGGCGGC
  CTGCCATCAACTACTCGGTGTACGGGGAGGCAGCACAGGTCAGGAGGCGCCGCTTTGAGCGTGCTGTTGG
  ACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATGAGAGTTTCTTATCAAGACAAAGGT
  TTCCTCCACAATGAAGTCAAAGTGAGTACAATGTGGAGAGGGTTTCCAAATGTTGTTACTTCAGCGATAA
  CACTGCCTAACATTAGGAAACCTGATGGCTATGATTACTACGCCTTTTCCAAAGATCAATACTATAACAT
  TGATGTGCCTACAAGAACAGCAAGAGCAATTACCACACGTTCAGGGCAGACCCTCTCCAAAATCTGGTAC
  AACTGTCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110146
• Insert Size: 1272 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110146.1, NP_001103616.1
• RefSeq Size: 1884 bp
• RefSeq ORF: 1272 bp
• Locus ID: 96875
• UniProt ID: Q9JM99
• Cytogenetics: 1 G1
• Gene Summary: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks internal coding exons and segments, as compared to variant 1. The resulting isoform (2) is much shorter but has identical N- and C-termini, compared to isoform 1.