Pag1 (NM_053182) Mouse Untagged Clone

CAT#: MC212008

Pag1 (untagged) - Mouse phosphoprotein associated with glycosphingolipid microdomains 1 (Pag1), transcript variant B, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pag1
• Synonyms: Cbp; F730007C19Rik; Pag
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212008 representing NM_053182
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCTGCAGGAAGCGTACTGAGCAGTGGGCAGATGCAGATGCAGATGGTCCTGTGGGGAAGTCTGG
  CTGCCGTGGCCATGTTCTTCCTCATCACCTTCCTCGTCCTCCTGTGTTCCACTTGCGACAGAGAGAAGAA
  GCCACGGCAGCATAGCGGGGACCATGAGAACCTGATGAATGTGCCTTCCGACAAGGACATGTTCAGCCAT
  TCGGCAACCAGCCTGACAACAGATGCACTGGCTAGCAGCGAACAGAACGGGGTGCTCACCAATGGCGACA
  TTCTTTCAGAAGACAGCACGCTGACCTGCATGCAGCACTATGAGGAAGTCCAGACCTCGGCTTCCGATCT
  CCTGGACTCCCAGGACAGCACAGGAAAGGCCAAGTGCCACCAGAGCCGGGAGCTGCCCAGGATCCCGCCT
  GAGAACGCCGTGGACGAGATTCTCACAGCCAGGGCTGCAGACACGGAACTGGGGCCCGGAGTGGAGGGGC
  CCTACGAGGTGCTCAAGGACAGCTCCTCCCAGGAGAACATGGTGGAGGACTGTCTGTATGAAACAGTGAA
  GGAGATCAAGGAAGTGGCAGACAAAGGCCAGGGTGGCAAGTCCAAGTCCACTTCTGCCTTGAAGGAGCTT
  CAGGGGGCTCCCATGGAGGGCAAGGCCGACTTTGCTGAATATGCCTCTGTGGACAGAAACAAAAAGTGCC
  GCCACAGCGCGAACGCAGAGAGCATTCTGGGAACTTGCAGTGACCTGGACGAGGAAAGCCCACCTCCTGT
  CCCGGTTAAGCTTCTGGATGAGAATGCCAACCTTCCCCAGGAGGGAGGAGGACAGGCAGAGGAGCAAGCT
  GCAGAAGGGACCGGTGGACACAGCAAGAGATTTAGTTCCTTGTCATACAAGTCTCGGGAAGAAGACCCGA
  CTCTTACAGAAGAGGAGATCTCAGCAATGTATTCCTCAGTGAATAAGCCTGGCCAGTCGGCACACAAACC
  TGGACCATGTATGAAAGGGCCAGAATCTGCCTGCCACTCCATGAAGGGCCTCCCTCAGAGGTCGTCATCT
  TCCTGCAATGACCTCTATGCCACTGTGAAAGACTTTGAGAAAACTCCCAACAGCATCAGCACACTGCCCC
  CGGCAAGGAGACCCAGTGAGGAGCCAGAGCCTGACTATGAAGCCATACAGACTCTAAACAGAGAGGATGA
  GAAGGTCCCTCTGGAGACCAATGGCCACCATGTCCCCAAGGAGAGTGACTACGAGAGCATTGGGGACTTG
  CAGCAATGCAGAGATGTCACCAGGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053182
• Insert Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053182.5, NP_444412.2
• RefSeq Size: 8197 bp
• RefSeq ORF: 1290 bp
• Locus ID: 94212
• UniProt ID: Q3U1F9
• Cytogenetics: 3 A1
• Gene Summary: Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (B) differs in the 5' UTR compared to variant A. Both variants A and B encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.