Trim7 (NM_053166) Mouse Untagged Clone

CAT#: MC212007

Trim7 (untagged) - Mouse tripartite motif-containing 7 (Trim7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim7
• Synonyms: AI790312
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC212007 representing NM_053166
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGAGAAAGAGAAGGTAGGGGCGGAGTTCCAAGCGCTGCGGGCCTTCCTGGTGGAGCAGGAGG
  GTCGGCTTCTAAGCCGACTGGAGGTGCTGTCCCGGGAAGTGACACAGAAACAGAATGAGAACCTGGCCCA
  GCTGGAGGGTGAGATCACTCAGCTGTCCAAACTCAGCGGCCAGATCCAGGAGACAGCTCAGAAGCCGGAC
  CTAGACTTCCTCCAGGAGTTCAAAAGCACACTGAGCAAGTGCAGCAGCGTGCCCAGCTCCAAGCCAACCA
  CAGTCTCATCTGAGATGAAGAATAAAGTGTGGAATGTTTCCCTCAAGAGTTTCGTCTTAAAAGGATTGCT
  GAAGAAGTTCAAAGAGGATCTCCAGGGAGAGCTGGAGAAAGAAGAGAAAGTGGAACTCACTTTGGATCCA
  GACACAGCCAACCCCCGTCTCATCTTGTCCCTGGATCTAAAGAGCGTACGTCTAGGACAGCGCGCCCAGG
  ACTTGCCTAACCATCCTCGCCGCTTTGATACCAACACCCGCGTTCTGGCATCCTGTGGTTTCTCCTCTGG
  GCGACATCACTGGGAGGTGGAAGTGGGCTCCAAGGACGGCTGGGCTTTCGGTGTGGCCCGGGAGAGCGTG
  CGTCGCAAGGGCCTCACGCCTTTTACCCCCGAGGAGGGCGTCTGGGCAATGCAACTCAACAATGGGCAAT
  ACTGGGCGGTGACCAGCCCCGAGAGGACACAGCTCAACTGTGGGCACTTGTCGCGGGTGAGGGTGGCGCT
  GGACCTTGAGGTGGGAGCAGTGTCCTTCTATGCAGTGGAGGACATGCGCCACCTCTACACCTTCCGCGTC
  AACTTCCAGGAGCGAGTGTTCCCCCTTTTCTCTGTTTGTTCTACCGGCACATACTTGAGAATCTGGCCTT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053166
• Insert Size: 912 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053166.2, NP_444396.2
• RefSeq Size: 1366 bp
• RefSeq ORF: 912 bp
• Locus ID: 94089
• UniProt ID: Q923T7
• Cytogenetics: 11 B1.2
• Gene Summary: E3 ubiquitin-protein ligase (By similarity). Mediates 'Lys-63'-linked polyubiquitination and stabilization of the JUN coactivator RNF187 in response to growth factor signaling via the MEK/ERK pathway, thereby regulating JUN transactivation and cellular proliferation (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 1, which is shorter at the N-terminus, compared to isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.