Gatsl2 (NM_030719) Mouse Untagged Clone

CAT#: MC211937

Gatsl2 (untagged) - Mouse GATS protein-like 2 (Gatsl2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gatsl2
• Synonyms: 7530428J21Rik; AW742413; Gats; Gatsl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211937 representing NM_030719
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGCACATTCTGGAACACCGGCTGCAAGTTGCCAGCGTCGCCAAGGAGAGTATCCCGCTCTTCA
  CCTACGGCCTCATCAAACTTGCCTTCCTGTCTTCCAAGACCAGGTGCAAGTTTTTCAGTCTGACCGAGAC
  CCCTGAGGACTACACCATCATCGTAGATGAGGAGGGTTTCCTTGAGCTACCCTCCTCAGAGCACCTGAGT
  GTGGCTGATGCCACCTGGCTGGCCCTGAACGTGGTGTCCGGTGGCGGCAGCTTCTCCAGCTCCCAGCCCA
  TCGGTGTGACAAAGATTGCCAAGTCTGTCATTGCCCCACTCGCCGACCAGAACATCTCCGTGTTTATGCT
  GTCCACCTATCAAACTGACTTCATCCTGGTGCGGGAGCGGGACCTGCCCTTCGTCACTCACACCTTGTCC
  TCGGAGTTCACCATCCTACGCGTTGTCAATGGAGAAACGGTGGCGGCTGAGAATCTCAGCTTCACCAATG
  GCTTTGTGAAGCCCAAGATGGTCCAGAGGCCAGTCATCCACCCACTGTCTAGCCCAAGCAACAGGTTCTG
  TGTCACCAGCCTGGACCCTGACACACTGCCCGCCGTCGCCACCCTTCTCATGGATGTGATGTTCTACTCC
  AATGGAGTGAAGGACCCCATGGCTGCCAGTGACGATTGTGGCCACATCCGCTTCTTCTCCTTCTCACTCA
  TTGAGGGCTACATCTCACTGGTGATGGACGTACAGACCCAGCAGAGGTTCCCGAGTCACTTGCTATTCAC
  GAGTGCATCTGGAGAGCTCTGGAAGATGGTGCGCATAGGAGGACAACCCTTGGGATTTGATGAATGTGGG
  ATTGTAGCCCAGATCTCAGAGCCCTTGGCAGCTGCAGACATCCCAGCATATTACATCAGTACTTTCAAGT
  TCGACCATGCATTGGTTCCAGAAGAGAATATCAGTGGGGTTATCCATGCCCTGAAGGTCAGTCAAGCAGG
  GAAGCATTAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_030719
• Insert Size: 990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030719.3, NP_109644.2
• RefSeq Size: 4807 bp
• RefSeq ORF: 990 bp
• Locus ID: 80909
• UniProt ID: Q8CAB8
• Cytogenetics: 5 G2
• Gene Summary: Functions as a negative regulator of the TORC1 signaling pathway through the GATOR complex. As part of homodimers or heterodimers with CASTOR1, directly binds and inhibits the GATOR subcomplex GATOR2 and thereby mTORC1. Does not directly bind arginine, but binding of arginine to CASTOR1 disrupts the interaction of CASTOR2-containing heterodimers with GATOR2 which can in turn activate mTORC1 and the TORC1 signaling pathway.[UniProtKB/Swiss-Prot Function]