Rhoj (NM_023275) Mouse Untagged Clone

CAT#: MC211926

Rhoj (untagged) - Mouse ras homolog gene family, member J (Rhoj), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rhoj
• Synonyms: 1110005O19Rik; Arhj; AW210585; TC10L; TCL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211926 representing NM_023275
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCAGAGAGAGGACCGACAGCAGCTGCGGCTGCAATGGACATGAGGAGAACAGGATCCTGAAGT
  GCGTGGTGGTCGGGGACGGCGCGGTGGGGAAGACCTGCTTGCTGATGAGCTACGCCAACGACGCCTTCCC
  AGAGGAATACGTGCCCACTGTGTTTGACCACTACGCAGTTACCGTGACGGTGGGAGGCAAGCAGCACTTG
  CTTGGACTATACGACACTGCAGGACAGGAGGATTACAACCAGCTGCGGCCACTCTCTTACCCCAACACAG
  ATGTGTTTCTCATCTGCTTCTCTGTCGTAAACCCAGCCTCTTACCACAACGTTCAGGAGGAGTGGGTCCC
  GGAACTGAAGGACTGCATGCCTCATGTGCCTTATGTGCTCATCGGAACCCAGATTGATCTCCGAGATGAC
  CCCAAAACCTTGGCGCGTCTGCTGTACATGAAGGAGAAGCCCCTCACCTATGAGCACGGCGTGAAGCTGG
  CGAAAGCGATTGGAGCGCAGTGCTACTTGGAATGTTCTGCCCTGACCCAGAAGGGTCTCAAAGCAGTTTT
  TGATGAAGCCATCCTCACCATTTTCCACCCCAAGAAAAAAAAGAAAGGCTGCTTAGGGTGTCACGGCTGC
  TGTGCAATTATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023275
• Insert Size: 645 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023275.2, NP_075764.1
• RefSeq Size: 2358 bp
• RefSeq ORF: 645 bp
• Locus ID: 80837
• UniProt ID: Q9ER71
• Cytogenetics: 12 C3
• Gene Summary: Plasma membrane-associated small GTPase specifically involved in angiogenesis (By similarity). Required for endothelial cell migration during vascular development via its interaction with GLUL (By similarity). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:10967094).[UniProtKB/Swiss-Prot Function]