Pofut2 (NM_030262) Mouse Untagged Clone

CAT#: MC211921

Pofut2 (untagged) - Mouse protein O-fucosyltransferase 2 (Pofut2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pofut2
• Synonyms: 2310011G23Rik; AI256847; BC003494; C21orf80; FUT13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211921 representing NM_030262
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTCAGCGTCGTCTGCCTGCTGCTGGCAGCGGCATCCTGGCGGCCGGTCTCGGCTTCAGGAG
  AAGAATTCTGGCCTGGCCAGTCGGCGGCGGACATCCTGTCGGGGGCGGCATCCCGCAGACGGTACCTCCT
  GTATGATGTCAACCCCCCAGAAGGCTTCAATCTCCGCAGGGATGTCTATATCCGGGTGGCATCCTTGCTG
  AAGACGCTGTTGAAGACTGAAGAGTGGGTGCTAGTCCTGCCCCCGTGGGGCCGCCTCTACCACTGGCAAA
  GCCCTGACATCCACCAGGTCCGGATTCCCTGGTCCGAGTTTTTTGACCTCCCGAGTCTCAATAAAAACAT
  CCCCGTTATAGAGTATGAGCAGTTCATTGCAGAGTCCGGCGGGCCCTTTATCGACCAGGTGTACGTCCTA
  CAAGGTTACGCCGAGGGCTGGAAGGAGGGCACCTGGGAGGAGAAGGTGGACGCACGGCCCTGTATTGACC
  CTCTGCTGTACTCGCAGGACAAGCATGAGTATTACAGAGGCTGGTTTTGGGGCTACGAAGAAACACGGGG
  TCTGAATGTCTCCTGCCTGTCCGTCCAGGGGTCGGCTTCCATCGTCGCGCCTGTCCTCTTAAAAAACACA
  TCTGCTCGGTCTGTGATGCTGGACCGAGCTGAGAATCTGCTTCATGACCACTATGGAGGCCGGGAGTACT
  GGGATACCCGGCGCAGCATGGTGTTTGCAAAGCACTTGCGGGCTGTGGGAGACGAGTTTAGGAGCCAGCA
  CCTCAACTCGACGGATGCCGCTGATAAGATGGCCCCGGAGGAAGACTGGACGAAGATGAAGGTCAAACTG
  GGCTCAGCACTAGGCGGCCCCTACCTCGGAGTCCACCTGAGAAGGAAGGACTTCATCTGGGGCCACAGGG
  AGGATGTGCCCAGCCTGGAGGGCGCCGTGAAGAAGATCCGCAGCCTCATGAAGACTCATCAGCTGGACAA
  GGTGTTCGTGGCCACAGACGCCATCAGGAAGGAGCAGGAAGAATTGAGGAAGCTGCTGCCGGAAATGGTG
  AGGTTTGAGCCCACGTGGGAGGAGCTGGAGCTGTACAAGGACGGAGGCGTCGCCATCATCGATCAGTGGA
  TCTGCGCTCATGCCAGGTTTTTTATTGGCACCTCTGTTTCCACATTTTCCTTTCGGATTCATGAAGAGAG
  AGAGATCCTGGGGTTGGACCCCAAGACAACATACAACCGGTTTTGTGGAGACCAGGAGAAAGCATGTGAG
  CAGCCCACACACTGGAAGATTGCGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030262
• Insert Size: 1290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030262.3, NP_084538.2
• RefSeq Size: 2219 bp
• RefSeq ORF: 1290 bp
• Locus ID: 80294
• UniProt ID: Q8VHI3
• Cytogenetics: 10 C1
• Gene Summary: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity). O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.[UniProtKB/Swiss-Prot Function]