Bhlhe41 (NM_024469) Mouse Untagged Clone

CAT#: MC211916

Bhlhe41 (untagged) - Mouse basic helix-loop-helix family, member e41 (Bhlhe41), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bhlhe41
• Synonyms: 6430520M22Rik; Bhlhb; Bhlhb2l; Bhlhb3; DEC2; Sh; Sharp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211916 representing NM_024469
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAAGGAATCCCTCATTTGCAAGAGAGACAGTTACTGGAACATAGGGATTTTATAGGACTGGACT
  ATTCCTCTTTGTATATGTGTAAACCCAAAAGGAGCTTGAAGCGAGACGATACCAAGGATACCTACAAGTT
  ACCGCACAGATTAATAGAAAAGAAGAGACGAGACCGAATTAATGAATGCATTGCTCAGCTGAAAGATTTA
  CTGCCCGAACATCTGAAATTGACAACACTGGGGCATTTGGAGAAAGCAGTAGTCTTGGAATTAACTTTAA
  AGCACTTGAAAGCGCTAACAGCCTTAACTGAGCAGCAGCATCAGAAGATAATTGCTTTACAGAATGGGGA
  GCGCTCTCTGAAATCGCCGGTCCAGGCCGACTTGGATGCGTTCCACTCGGGGTTTCAAACCTGCGCCAAA
  GAAGTCTTGCAATACCTCGCGCGCTTTGAGAGCTGGACACCCAGGGAGCCGCGCTGCGCACAGCTCGTCA
  GCCACCTGCATGCCGTGGCCACCCAGCTCCTGACGCCACAGGTGCCCTCCGGCAGGGGCTCTGGGCGCGC
  GCCCTGCAGCGCGGGGGCTGCGGCCGCCTCGGGTCCCGAGCGCGTCGCCCGCTGCGTGCCGGTCATCCAG
  CGGACTCAGCCCGGCACGGAGCCGGAACACGACACGGACACCGACAGCGGCTACGGAGGCGAGGCGGAGC
  AGGGCCGCGCGGCCGTCAAGCAGGAGCCACCCGGGGACTCGTCGCCTGCGCCCAAGAGGCCGAAGCTGGA
  GGCGCGCGGCGCGCTCCTGGGCCCGGAGCCCGCGCTGCTCGGCTCGCTCGTGGCGCTTGGCGGGGGCGCG
  CCCTTCGCGCAGCCCGCTGCCGCGCCCTTCTGCCTGCCCTTCTATCTGCTGTCGCCGTCCGCCGCCGCCT
  ACGTACAGCCCTGGCTAGACAAGAGCGGCCTGGACAAGTATCTGTACCCCGCGGCGGCCGCGCCCTTCCC
  GCTGCTGTATCCCGGCATCCCAGCAGCGGCCGCCGCTGCTGCTGCCGCCGCTTTCCCTTGCTTGTCGTCC
  GTGCTGTCGCCACCCCCGGAGAAGGCCGGCGCGACCGCCGGTGCCCCGTTCCTGGCGCACGAGGTGGCGC
  CCCCGGGGCCGCTGCGCCCCCAGCACGCGCATAGCCGCACCCACCTGCCGCGCGCTGTGAACCCGGAGAG
  CTCTCAGGAAGATGCCACGCAGCCGGCCAAGGACGCCCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_024469
• Insert Size: 1233 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024469.2, NP_077789.1
• RefSeq Size: 5995 bp
• RefSeq ORF: 1233 bp
• Locus ID: 79362
• UniProt ID: Q99PV5
• Cytogenetics: 6 77.7 cM
• Gene Summary: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with Arntl or compete for E-box binding sites in the promoter of Per1 and repress Clock/Arntl's transactivation of Per1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.