Spsb3 (NM_001163751) Mouse Untagged Clone

CAT#: MC211912

Spsb3 (untagged) - Mouse splA/ryanodine receptor domain and SOCS box containing 3 (Spsb3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spsb3
• Synonyms: 2310012N15Rik; 3300001M01Rik; mKIAA4204; SSB3; Tce1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211912 representing NM_001163751
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCGGGGGCGGGGATTGTTTGCGTCGCGGCCGGAACGGAAAGTGGGTCAGCGCCGGGGGAGCTT
  TTTGGCCGCAGGTTCTCTCCACTATGGCCCGGCGTACACGGAGTAGCAGGGCATGGCATTTCGTCCTGAG
  CGCAGCCCGCCGAGATACAGATGCCCGGGCTGTGGCTCTGGCAGGCAGCTCTAACTGGGGCTATGACTCT
  GATGGGCAGCACAGCGACTCCGACTCTGACCCTGAGTACTCCTCCCTGCCACCATCCATCCCCAGTGCTG
  TGCCTGTGACAGGAGAGTCCTTCTGTGACTGCGAGGGCCAGAATGAGGCTACCTTCTGCAACAGTCTGCA
  CACAGCACACCGTGGCAAGGACTGCCGGTGTGGTGAGGAGGATGAGGATTTTGATTGGGTCTGGGATGAC
  CTGAACAAGTCCTCAGCCACCTTGCTGAGCTGTGATAATCGCAAGGTCAGCTTTCACATGGAGTATAGCT
  GTGGCACTGCAGCCATTCGGGGCACCAAGGAGCTAGGGGATGGCCAACACTTCTGGGAAATCAAGATGAC
  CTCTCCAGTGTATGGCACAGACATGATGGTGGGCATTGGGACGTCGGATGTAGACCTGGACAAGTACCAC
  CACACGTTCTGCAGCCTGCTGGGCAGGGATGAGGACAGCTGGGGGCTCTCCTACACTGGGCTCCTCCACC
  ACAAAGGTGACAAGACGAGCTTCTCTTCACGCTTTGGCCAGGGCTCCATTATTGGTGTGCACTTGGACAC
  CTGGCATGGGACACTGACATTCTTCAAGAACAGGAAGTGCATAGGAGTGGCTGCTACTCGGCTTCAAAAC
  AGAAGGTTCTACCCGATGGTTTGCTCCACGGCTGCCAAGAGCAGCATGAAAGTCATCCGCTCCTGCGCCA
  GCTCCACATCCCTGCAGTACCTGTGCTGCTACCGCTTGCGCCAACTGCGGCCAAATTCAGGGGACACCCT
  CGAGGGCCTGCCCTTGCCACCTGGCCTCAAGCAAGTGCTGCATAACAAGCTGGGCTGGGTTCTGAGCATG
  AACTGCAACCACTGGAAATCCCCTGTGCCCCCTCCTGGCACAGCTACACCTGGTGCCGAGAGTCTGGAGA
  CCAGGCCCTGTCAGAGGAAGCGCTGCCGAAGAAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163751
• Insert Size: 1158 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163751.1, NP_001157223.1
• RefSeq Size: 1608 bp
• RefSeq ORF: 1158 bp
• Locus ID: 79043
• UniProt ID: Q571F5
• Cytogenetics: 17 A3.3
• Gene Summary: May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.