Srd5a1 (NM_175283) Mouse Untagged Clone

CAT#: MC211909

Srd5a1 (untagged) - Mouse steroid 5 alpha-reductase 1 (Srd5a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Srd5a1
• Synonyms: 0610031P22Rik; 4930435F02Rik; S5AR 1; Srd5a-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211909 representing NM_175283
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGGATGAGTTGCGCCTACTGGATGCGCTAGTCTACCTGGAGGGTTTCCTGGCTTTCGTGGCCT
  TCGTGGGGCTCCAGATGGTGGGCTCTTCCTACGGCCGCTACTCCTCGCAGTGGTCCGGCCGCCGAGTGCC
  CGCGCGACCTGCCTGGTTCCTACAGGAGCTGCCTTCAATGGCCTGGCCGCTGTACGAGTGCATCCGTCCT
  GCAGCCGCCCGACTGGGCAACCTGCCTAACCGCGTCCTGCTAGCTATGTTTCTGATCCACTACGTGCAAA
  GGACGCTGGTTTTTCCAGTTCTGATCAGGGGAGGGAAGCCCACCCTGCTGTTCACCTTTGTCTTGGCCTT
  CCTGTTCTGCACCTTAAATGGCTACTTGCAGAGCCGATACTTGAGCCAGTTTGCGGTGTATGCTGAAGAC
  TGGGTAACCCATCCCTGTTTCCTGACAGGCTTTGCCCTGTGGTTAGTGGGCATGGTGATAAATATCCACT
  CAGACCACATCCTGCGGAATCTGAGAAAACCAGGGGAAACTGGATACAAAATACCCAGGGGAGGCCTGTT
  TGAATATGTATCTTCAGCCAACTATTTTGGGGAGCTCGTGGAGTGGTGTGGCTTTGCACTGGCCAGCTGG
  TCCCTCCAGGGCGTGGTGTTTGCTCTGTTCACCCTGTGTGCACTGTTCACCAGAGCGAGGCAGCATCATC
  AGTGGTACCTCGAGAAGTTTGAAGATTACCCCAAAACAAGAAAAATACTAATTCCATTCCTGCTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175283
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175283.3, NP_780492.2
• RefSeq Size: 2681 bp
• RefSeq ORF: 768 bp
• Locus ID: 78925
• UniProt ID: Q68FF9
• Cytogenetics: 13 35.55 cM
• Gene Summary: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology (By similarity).[UniProtKB/Swiss-Prot Function]