Pde6h (NM_023898) Mouse Untagged Clone

CAT#: MC211893

Pde6h (untagged) - Mouse phosphodiesterase 6H, cGMP-specific, cone, gamma (Pde6h), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde6h
• Synonyms: A930033D18Rik; PDEgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211893 representing NM_023898
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACAGCCCTTCCCTGAGTCCTCCAGCACCAAGCCAGGGCCCTACTACCCCACGCAAAGGGCCCC
  CCAAGTTCAAGCAGAGGCAGACTCGACAGTTCAAGAGCAAGCCTCCCAAGAAAGGGGTGAAAGGGTTTGG
  AGATGACATCCCAGGCATGGAGGGGCTAGGAACAGATATCACGGTCATCTGTCCCTGGGAAGCGTTCAGC
  CATCTGGAGCTGCATGAGCTTGCTCAGTTCGGGATCATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023898
• Insert Size: 252 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023898.4, NP_076387.2
• RefSeq Size: 574 bp
• RefSeq ORF: 252 bp
• Locus ID: 78600
• UniProt ID: P61249
• Cytogenetics: 6 G1
• Gene Summary: Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.[UniProtKB/Swiss-Prot Function]