Htra3 (NM_001042615) Mouse Untagged Clone

CAT#: MC211891

Htra3 (untagged) - Mouse HtrA serine peptidase 3 (Htra3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Htra3
• Synonyms: 2210021K23Rik; 9530081K03Rik; Prsp; Tasp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211891 representing NM_001042615
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGCGCGCGCTGCTCCCCGCCACGCTGGCCATTCTGGCCACGCTGGCTGTGTTGGCTCTGGCCC
  GGGAGCCCCCAGCGGCTCCGTGTCCTGCGCGCTGCGACGTGTCGCGCTGTCCGAGCCCTCGCTGCCCTGG
  GGGCTATGTGCCTGACCTCTGCAACTGCTGCCTGGTGTGCGCTGCCAGCGAGGGCGAGCCCTGCGGCCGC
  CCCCTGGACTCTCCGTGCGGGGACAGTCTGGAGTGCGTGCGCGGCGTGTGCCGCTGCCGTTGGACCCACA
  CTGTGTGTGGCACAGACGGGCATACTTATGCCGACGTGTGCGCGCTGCAGGCCGCCAGCCGTCGTGCGTT
  GCAGGTCTCCGGGACTCCAGTGCGCCAGCTGCAGAAGGGTGCCTGTCCCTCTGGTCTCCACCAGCTGACC
  AGTCCGCGGTACAAGTTCAACTTCATCGCCGATGTGGTGGAGAAGATTGCGCCAGCTGTGGTCCACATAG
  AGCTCTTTCTGAGACACCCCCTGTTTGGCCGGAATGTGCCGCTGTCCAGTGGCTCGGGCTTCATCATGTC
  AGAAGCCGGTTTGATCGTCACCAACGCCCACGTGGTCTCCAGCTCCAGCACTGCCTCCGGCCGGCAGCAG
  CTGAAGGTGCAGCTGCAGAATGGGGATGCCTATGAGGCCACCATCCAGGACATCGACAAGAAGTCGGACA
  TTGCCACGATTGTAATCCACCCCAAGAAAAAGCTCCCTGTGTTGCTGCTGGGTCACTCAGCAGACCTGCG
  GCCTGGCGAGTTCGTGGTGGCCATCGGCAGCCCCTTTGCCCTGCAGAACACCGTGACAACGGGCATTGTC
  AGCACTGCCCAGCGGGATGGCAAGGAGCTGGGTCTCCGGGACTCAGACATGGACTATATCCAGACCGATG
  CCATCATCAATTACGGGAACTCAGGAGGACCCCTGGTGAACCTGGATGGCGAGGTCATCGGCATCAACAC
  GCTCAAGGTTGCAGCTGGCATCTCCTTTGCCATCCCCTCAGATCGCATCACACGCTTCCTCTCTGAGTTC
  CAAAACAAGCATGTGAAAGCCCTCTCACCAGCACTGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042615
• Insert Size: 1092 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042615.2, NP_001036080.1
• RefSeq Size: 2209 bp
• RefSeq ORF: 1092 bp
• Locus ID: 78558
• UniProt ID: Q9D236
• Cytogenetics: 5 B3
• Gene Summary: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (PubMed:15206957). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform b which is shorter and has a distinct C-terminus, compared to isoform a.