Skap1 (NM_001177899) Mouse Untagged Clone

CAT#: MC211886

Skap1 (untagged) - Mouse src family associated phosphoprotein 1 (Skap1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skap1
• Synonyms: 1700091G21Rik; Scap1; Skap-55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211886 representing NM_001177899
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCTGTCGCCCTCCCGGAGGAGATCTGTTGGCTCCTGGAAGATACCGAAGACTTCCTGGCAGAAG
  GCTTGCAGAATGAGAACCTCAGCCCTGGGGCACAGGATCAGAGAGCCCACATTCTTCGGGGTTTCCAGCA
  AATCAAATCCAGGTACTGTTGGGATTTTCAGCCCCAAGGAGGTGACCTTGGCCAGGACGGCTCTGATGAC
  AATCTGAGTGGGACTCACGGCCCACCCCTCACGTCAGAAGCATCCTTTTGGTCAGATTATCAGGACGAGG
  GAATAGAAGACATCTTAAGAGGGGCTCAAGAGCTGGATAGCGTCATCAAGCAAGGGTACCTGGAGAAGAA
  AAGCAAAGATCATAGCTTCTTTGGGTCGGAGTGGCAGAAGCGCTGGTGTGTTATCAGCAGAGGGCTCTTC
  CTCTACTATGCTAATGAGAAAAGCAAGCAGCCCAAAGGGACCTTCCTCATTAAGGGCTACAGTGTCCGGA
  TGGCCCCTCACCTGCGAAAAGATTCCAAGAAAGAATCCTGCTTTGAACTGATCTCCCAGGATAGGCGCAG
  CTATGAGTTTACAGCTTCTAGTCCAGCTGAAGCCAGAGACTGGGTGGATCAAATAAGTTTCTTGTTAAAA
  GATCTTAGCTCCTTAACCATTCCCTTCGAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAGAAAGAAGAGG
  AAGAAATGTACAATGACGTCGATGGCTTTGACTCCCCAAGGTCTGGCTCCCAGTGCAGAGCCATGGCCTT
  GCCTGAGCCTACTGAGAAAGAGGAGGACATTTATGAAGTCCTACCAGTGGACTATGCCGATTATTACCAG
  GGCCTATGGGATTGCCATGGTGACCAGCCGGATGAACTGTCCTTCCAACGGGGTGACCTCATCCGCATTC
  TGAGCAAGGAGTACAACATGTACGGCTGGTGGGTAGGAGAGCTGAACAGCGTCATTGGAATTGTTCCAAA
  GGACTATCTCACCACCGCCTTTGAAATGGAAGGAATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177899
• Insert Size: 1020 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177899.1, NP_001171370.1
• RefSeq Size: 2142 bp
• RefSeq ORF: 1020 bp
• Locus ID: 78473
• UniProt ID: Q3UUV5
• Cytogenetics: 11 D
• Gene Summary: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate, in-frame exon, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1.