Spopl (NM_001165998) Mouse Untagged Clone

CAT#: MC211785

Spopl (untagged) - Mouse speckle-type POZ protein-like (Spopl), transcript variant 3, (10ug)


  "NM_001165998" in other vectors (4)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Spopl
Synonyms 4921517N04Rik; AU014935; BB233739; E430033K04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211785 representing NM_001165998
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAAAAGGGAAGAAACAAAAGCAATGGAAAGCCAAAGAGCATATCGATTTGTACAGGGGAAGGACTG
GGGATTTAAAAAATTCATTAGAAGGGATTTTTTGCTTGATGAAGCTAATGGTCTCCTTCCAGATGACAAG
CTTACGTTATTCTGTGAGCATCTCCAACACTGCTTGCAGAAGAGGTGGAAGCATTGCACTGCCCTCCCTA
AAGAACTGGCACCTCCCCCTCCACTGCAAGTGGTGCTGTAGACAGGTGAGTGTGGTCCAAGATTCAGTAA
ATGTATCAGGACACACTAGTACAAACACTTTGAAGGTGCCAGAATGTCGACTAGCAGAAGATTTAGGTAA
TCTCTGGGAAAACACAAGATTTACAGATTGCTGCTTTTTTGTGAGAGGAAAAGAATTCAAAGCTCATAAG
TCTGTGCTTGCAGCTCGATCCCCAGTTTTTAATGCAATGTTTGAACATGAAATGGAAGAATGCACAAAGA
ATCGAGTGGAAATAAATGATTTAGACCCTGAGGTATTTAAAGAAATGATGAGATTTGTTTACACAGGAAA
AGCACCAAATCTTGATAAAATGGCTGACAACTTGTTGGCAGCTGCAGACAAATATGCACTGGAACGGCTG
AAGGTCATGTGTGAAGAAGCTTTGTGTAGTAACCTCTCAGTAGAAAATGTTGCTGATACCCTTGTCCTTG
CAGATTTGCACAGTGCAGAACAGTTGAAAGCCCAGGCAATAGACTTTATTAATAGGTGCAGTGTTCTTCG
ACAACTTGGGTGTAAGGATGGGAAAAACTGGAACAACAACCAAGCAACTGACATAATGGAAACATCGGGG
TGGAAGTCCATGATTCAGTCTCATCCCCATTTAGTGGCAGAAGCGTTCCGAGCACTAGCATCTTCACAGT
GTCCACAGTTTGGTATTCCACGCAAAAGGTTAAAGCAGTCTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001165998
Insert Size 954 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001165998.1, NP_001159470.1
RefSeq Size 2722 bp
RefSeq ORF 954 bp
Locus ID 76857
UniProt ID Q2M2N2
Cytogenetics 2 A3
Gene Summary Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2 and 3 both encode isoform b.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.