Spopl (NM_001165998) Mouse Untagged Clone
CAT#: MC211785
Spopl (untagged) - Mouse speckle-type POZ protein-like (Spopl), transcript variant 3, (10ug)
"NM_001165998" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Symbol | Spopl |
Synonyms | 4921517N04Rik; AU014935; BB233739; E430033K04Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC211785 representing NM_001165998
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAAAAGGGAAGAAACAAAAGCAATGGAAAGCCAAAGAGCATATCGATTTGTACAGGGGAAGGACTG GGGATTTAAAAAATTCATTAGAAGGGATTTTTTGCTTGATGAAGCTAATGGTCTCCTTCCAGATGACAAG CTTACGTTATTCTGTGAGCATCTCCAACACTGCTTGCAGAAGAGGTGGAAGCATTGCACTGCCCTCCCTA AAGAACTGGCACCTCCCCCTCCACTGCAAGTGGTGCTGTAGACAGGTGAGTGTGGTCCAAGATTCAGTAA ATGTATCAGGACACACTAGTACAAACACTTTGAAGGTGCCAGAATGTCGACTAGCAGAAGATTTAGGTAA TCTCTGGGAAAACACAAGATTTACAGATTGCTGCTTTTTTGTGAGAGGAAAAGAATTCAAAGCTCATAAG TCTGTGCTTGCAGCTCGATCCCCAGTTTTTAATGCAATGTTTGAACATGAAATGGAAGAATGCACAAAGA ATCGAGTGGAAATAAATGATTTAGACCCTGAGGTATTTAAAGAAATGATGAGATTTGTTTACACAGGAAA AGCACCAAATCTTGATAAAATGGCTGACAACTTGTTGGCAGCTGCAGACAAATATGCACTGGAACGGCTG AAGGTCATGTGTGAAGAAGCTTTGTGTAGTAACCTCTCAGTAGAAAATGTTGCTGATACCCTTGTCCTTG CAGATTTGCACAGTGCAGAACAGTTGAAAGCCCAGGCAATAGACTTTATTAATAGGTGCAGTGTTCTTCG ACAACTTGGGTGTAAGGATGGGAAAAACTGGAACAACAACCAAGCAACTGACATAATGGAAACATCGGGG TGGAAGTCCATGATTCAGTCTCATCCCCATTTAGTGGCAGAAGCGTTCCGAGCACTAGCATCTTCACAGT GTCCACAGTTTGGTATTCCACGCAAAAGGTTAAAGCAGTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001165998 |
Insert Size | 954 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001165998.1, NP_001159470.1 |
RefSeq Size | 2722 bp |
RefSeq ORF | 954 bp |
Locus ID | 76857 |
UniProt ID | Q2M2N2 |
Cytogenetics | 2 A3 |
Gene Summary | Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, but with relatively low efficiency. Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL are less efficient than ubiquitin ligase complexes containing only SPOP. May function to down-regulate the activity of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes that contain SPOP (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate coding exon compared to variant 1, that causes a frameshift. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 2 and 3 both encode isoform b. |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222823 | Spopl (tGFP-tagged) - Mouse speckle-type POZ protein-like (Spopl) transcript variant 3, (10ug) |
USD 530.00 |
|
MR222823 | Spopl (Myc-DDK-tagged) - Mouse speckle-type POZ protein-like (Spopl), transcript variant 3 |
USD 330.00 |
|
MR222823L3 | Lenti ORF clone of Spopl (Myc-DDK-tagged) - Mouse speckle-type POZ protein-like (Spopl), transcript variant 3 |
USD 630.00 |
|
MR222823L4 | Lenti ORF clone of Spopl (mGFP-tagged) - Mouse speckle-type POZ protein-like (Spopl), transcript variant 3 |
USD 630.00 |
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