Xrcc3 (NM_028875) Mouse Untagged Clone

CAT#: MC211524

Xrcc3 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Xrcc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211524 representing NM_028875
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTGGATCAGCTGGACCTAAACCCCCGAATTACTGCTGCGGTTAAGAGGGGCAGACTGAAGTCAG
  TGAAGGAGATTCTGTGCTACTCCGGACCAGACTTGCAGCGGCTCACCGGCCTGCCCAGCCACGATGTGCA
  GTGCCTGCTGAGAGCTGCCTCTCTACACCTTCGGGGAAGCCGCGTCCTCTCAGCACTGCATCTGTTCCAG
  CAGAAGGAGAGCTTCCCTGAGCAGCATCAACGCCTGAGCCTAGGCTGCCCTGTCCTGGATCAGTTCCTGG
  GTGGAGGCCTGCCCCTGGAGGGCATCACTGGCCTGGCTGGCTGCAGCTCAGCAGGAAAGACCCAGCTGGC
  GCTACAGCTCTGCCTGGCTGTGCAGTTCCCAAGACAATATGGAGGCCTAGAGGCCGGGGCTGTCTACATC
  TGCACAGAAGATGCCTTCCCCAGCAAGCGGCTGTGGCAGCTCATCGCGCAGCAGCGGAGGCTGCGGACAG
  ACGCGCCTGAGGAGCTGATCGAGAAGATCAGGTTCAGCAACCACATCTTCATTGAACACGCGGCCGACGT
  GGACACCTTGTTGGAGTGTGTGAGTAAGAAAGTCCCCATCCTGCTGTCGAGGGGCATGGCCCGCCTGGTG
  GTGGTTGACTCCATTGCAGCCCCATTTCGTTGTGAGTTCCACCTTCAGGCCTCAGCCATCAGGGCGAAGC
  TCCTGCTCTCGCTGGGGGCCACACTGCGAAGACTGAGCAGCACCTTCAGGAGCCCAGTGCTGTGCATCAA
  CCAGGTGACAGACATGGTGGAGGACCAGCAGTCTGTGTCCAGGTCACTAGGGGCTTCAGAAGAGCGCCTC
  TCTCCAGCCCTTGGCATCACCTGGGCTAACCAGCTCCTGATGCGACTGATGGTTGACCGCACCCATGAGG
  ACGATGTTACCACAGGCTTGCCCAGAAGCCCAGTTCGGACTCTCCGAGTGCTCTTCGCCCCCCACCTACC
  CCTCTCCTCCTGCTGCTACACAGTCAGTGGGGAAGGCATTAGGGGGATGCCAGGAACACAGTCCTACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028875
• Insert Size: 1050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028875.3, NP_083151.1
• RefSeq Size: 2479 bp
• RefSeq ORF: 1050 bp
• Locus ID: 74335
• UniProt ID: Q9CXE6
• Cytogenetics: 12 F1
• Gene Summary: This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Allelic variants in the human gene are associated with susceptibility to breast cancer and cutaneous malignant melanoma. [provided by RefSeq, Sep 2015]