Wdr33 (NM_001170967) Mouse Untagged Clone

CAT#: MC211521

Wdr33 (untagged) - Mouse WD repeat domain 33 (Wdr33), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Wdr33
• Synonyms: 1110001N06Rik; 2310011G05Rik; 2810021O11Rik; 8430413N20Rik; WDC146
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_001170967.1
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACAGAAATTGGCTCTCCTCCACGATTTTTCCATATGCCGAGGTTTCAACATCAGGCTCCCCGAC
  AATTATTTTATAAGCGACCTGATTTTGCACAGCAGCAAGCAATGCAGCAGCTCACCTTTGATGGAAAACG
  AATGAGAAAAGCTGTAAACCGAAAAACCATAGACTACAATCCCTCTGTAATTAAGTATTTGGAGAATAGG
  ATATGGCAAAGAGATCAGAGAGATATGCGGGCAATTCAGCCGGATGCAGGTTATTATAATGATCTGGTCC
  CACCAATAGGAATGTTGAATAATCCTATGAATGCAGTAACAACAAAATTTGTTAGGACATCAACAAACAA
  AGTTAAATGCCCAGTATTTGTTGTGAGGTGGACCCCGGAAGGAAGACGATTGGTCACTGGAGCTTCTAGT
  GGGGAGTTCACCTTGTGGAATGGGCTGACTTTCAACTTTGAAACCATATTGCAGGCTCATGATAGCCCTG
  TGAGGGCCATGACTTGGTCACATAATGACATGTGGATGTTGACAGCAGATCATGGAGGATATGTGAAATA
  TTGGCAATCCAACATGAACAACGTCAAGATGTTCCAGGCACATAAGGAGGCGATTAGAGAGGCCAGTTTC
  TCACCCACGGATAATAAATTTGCTACATGCTCTGATGACGGCACTGTTAGAATCTGGGATTTTCTTCGTT
  GCCATGAGGAAAGAATTCTCCGAGGGTTTCTCTTTGTAGCCCTGGCTGTCCTGGAAATTGCTCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170967
• Insert Size: 768 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170967.1, NP_001164438.1
• RefSeq Size: 1148 bp
• RefSeq ORF: 768 bp
• Locus ID: 74320
• Cytogenetics: 18 B1
• Gene Summary: Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has multiple differences, compared to variant 1. The encoded isoform (3) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.