Hvcn1 (NM_001042489) Mouse Untagged Clone

CAT#: MC211484

Hvcn1 (untagged) - Mouse hydrogen voltage-gated channel 1 (Hvcn1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hvcn1
• Synonyms: 0610039P13Rik; AI450555; BTS; HV1; mVSOP; Vsop.
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211484 representing NM_001042489
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCCCATGACCCAAAGGCCGTCACTCGCAGAACCAAGGTGGCTCCCACCAAGAGGATGAGCAGGT
  TCTTGAAACACTTTACGGTGGTTGGGGACGACTACCACACGTGGAATGTCAACTACAAGAAGTGGGAGAA
  TGAGGAGGAGGAGGAGGAGCCAGCGCCCACATCAGCAGAGGGTGAGGGCAATGCTGAGGGCCCAGATGCC
  GAGGCTGGCTCTGCCTCCACGCCCAGGCAGTCCCTGGACTTCAGGAGCCGACTGAGGAAACTCTTCAGTT
  CCCACAGGTTTCAGGTCATCATCATCTGCCTGGTGGTCCTGGACGCCCTCCTCGTGCTTGCTGAACTCCT
  CCTGGATTTGAAGATCATCGAGCCGGACGAGCAAGACTATGCGGTCACGGCGTTCCACTACATGAGCTTT
  GCCATCCTGGTCTTCTTCATGTTGGAGATTTTTTTCAAGATCTTCGTCTTCCGCTTAGAGTTCTTCCACC
  ACAAGTTTGAGATCCTGGATGCCTTCGTGGTGGTGGTGTCTTTCGTCCTTGACCTTGTCCTCTTGTTTAA
  AAGCCACCACTTCGAAGCTCTAGGGCTGCTGATCTTGCTTCGGCTCTGGAGGGTGGCCCGGATCATCAAT
  GGCATCATCATCTCCGTGAAGACACGCTCAGAACGGCAGATCTTAAGGCTAAAGCAGATAAATATCCAAC
  TGGCCACCAAGATCCAGCATCTGGAATTCAGCTGCTCCGAGAAGGAACAAGAAATTGAGCGGCTCAACAA
  GCTGTTGAAACAGAATGGACTTCTCGGGGACGTGAACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042489
• Insert Size: 810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042489.2, NP_001035954.1
• RefSeq Size: 2790 bp
• RefSeq ORF: 810 bp
• Locus ID: 74096
• UniProt ID: Q3U2S8
• Cytogenetics: 5 F
• Gene Summary: Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.