Whrn (NM_001008796) Mouse Untagged Clone

CAT#: MC211459

Whrn (untagged) - Mouse whirlin (Whrn), transcript variant 7, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Whrn
• Synonyms: 1110035G07Rik; AW122018; AW742671; bM340H1.8; C430046P22Rik; mKIAA1526; whirlinNT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211459 representing NM_001008796
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCGAGGCAGCCCCCCGGCCCCGGGGTTGGAGACACGTATTCCATGGTTTCCTACAGCGACACAG
  GCTCATCCACAGGCAGCCACGGCACCTCTACAACCGTCAGCTCGGCCAGGAATACATTAGACCTGGAAGG
  AACCGGTGAGACCACCCAGGGCAGCACCAACGCTCTCCCGGATGTGTCTGTGGATGATGTCAAATCCCCC
  TCAGAAGATCTGCCTGGTATCAAGCCACCTCCTCCCCCACCACCCCTGGCTCAAGGTCATGACCGCTTGC
  TTGGCCAGCCACGGAAGCCAGGGAGGGAGGACCCCGCACCTCTGTCTTCTGCTGCCCACTCAGGCATTGT
  CTTCTCAGCACCACGGAACCGCAGCCCGCCGCCAGGGACTGCTCCCACTCCGGGACCTTCCTCAGCACAG
  GACTCTCCCTCCTCCCCCATTTATGCCTCCATCTCCCATGCCAACCCCAGTTCCAGAAAGCCGCTGGACA
  CCCACCTGGCCCTGGTTAACCAGCACCCCATCGGCCCCTTCCCTCGAGTCCAGTCCCCACCTCACCTGAA
  GAGCCCTCCTGCAGAGACCCCAGGAGCTGGGGCCTGCCTTCCACCACCATCACCCTCTGAACACCCTGAC
  GCCGTGGGTGCAAACCAGCACTTTGTTCTGGTGGAGGTGCACCGTCCGGACAGTGAGCCTGATGTGAATG
  AAGTGCGGGCTCTGCCCCAGACTCGCACAGCCTCCACACTCTCTCAGCTCTCAGACAGTGGGCAGACCCT
  GAGCGAGGACAGTGGCGTGGATGCCGGGGAGACGGAGGCCAGCACCTCAGGCCGAGGCAGACAGACAGCA
  TCCGCTAAGAACAAGAATGGCAAGGAGCAGCCCCGGACGGAGAGGACCGCGGAGGGTGCCAACAAACCTC
  CCGGCCTGCTGGAGCCAACGTCCACCCTGGTCCGTGTGAGGAAAAGTGCAGCCACACTGGGCATCGCCAT
  TGAGGGCGGTGCCAACACACGCCAGCCTCTGCCCAGGATCGTCACAATTCAGCGAGGAGGTTCTGCCCAT
  AACTGTGGACAGCTCAAGGTGGGCCACGTAATTCTGGAAGTGAATGGGCAGACACTTCGGGGTAAGGAGC
  ACAAGGAGGCCGCCCGAATCATCGCTGAGGCCTTCAAGACCAAGGAGAGAGACTACATCGACTTTCTGGT
  CACTGAGTTCAACGTGATGCTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001008796
• Insert Size: 1215 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008796.1, NP_001008796.1
• RefSeq Size: 2419 bp
• RefSeq ORF: 1215 bp
• Locus ID: 73750
• Cytogenetics: 4 33.97 cM
• Gene Summary: This gene encodes a protein required for elongation and actin polymerization in the hair cell stereocilia. The encoded protein is localized to the cytoplasm and co-localizes with the growing end of actin filaments. Mutations in this gene have been linked to deafness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]