Ttc19 (NM_029704) Mouse Untagged Clone

CAT#: MC211361

Ttc19 (untagged) - Mouse tetratricopeptide repeat domain 19 (Ttc19), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ttc19
• Synonyms: 2010204O13Rik; 2810460C24Rik; AI505442
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC211361 representing NM_029704
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGGTTGCTTCGCTGGCGTCTGGGTCGAACCCTGCTGCGGGCCGCGGGCCGGCGGTGTGGGGGTT
  GTACGGCGCGCCTGCTGCCGGAGCGGACGGGCGACGCGGGGACGGGCGCGGAGCGGCTGCGGACCCGAGG
  CGCGCCGGCGCGGGGGCACGGGGTGCTGCCGCTGTTGGCAGCTCTCGCCTGGTTCTCGCGACCCGCGGCG
  ACGGCCGAGCAGCCGGGAGAGGACGCGTCAGACGAGGCGGAGGCCGAGATCATCCAGCTGCTGAAGCAAG
  CCAAGTTGAGCATCATGAAAGACGAGCCAGAAGCGGCCGAGCTGATTTTGCATGACGCTCTTCGCCTTGC
  GTATGAGAGTGACAACAGGAAGGCCATCACGTACACTTATGACCTGGCAGAACAGCTTTTTAAAGCAACG
  ATGAGTTATCTGCTTGGAGGAGGCATGAAGCAGGAAGACAATGCAATAATTGAAATCTCTCTGAAACTGG
  CCAACATCTATGCTGCTCAGAATAAACAGGAATTTGCCCTTGCTGGCTATGAATTCTGCATTTCAACTCT
  AGAGGGGAAAATTGAAAGAGAAAAGGAATTAGCAGAAGATATTATGTCAGAAGAAACAGCCAATACTTAC
  CTCCTCCTTGGTATGTGCTTAGACTCTTGTGCACGCTATCTGCTATTCTCCAAGCAGCTATCACAGGCAC
  AAAGGATGTATGAAAAAGCCCTGCAGATTTGTCAAGAAATACAAGGAGAAAGACACCCACAGACCATTGT
  GCTGATGAGTGACTTGGCTACTACTCTGGATGCACAGGGCCACTTTGATGATGCCTACATTTATATGCAG
  AGGGCGTCAGATCTGGCAAGAGAGATAAATCACCCTGAGCTGCACATGGTACTCAGCAATCTGGCTGCCA
  TTTTGATACACAGAGAGCGATACACACAAGCAAAGGAGATCTACCAGGAAGCGCTGAAGCGAGCAGAGCT
  GAAAAGAGATGAGGTTTCTGTTCAGCACATCAGGGAAGAATTGGCTGAACTGTCAAGAAAAAGTAGACGT
  TTGACTTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_029704
• Insert Size: 1059 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029704.2, NP_083980.2
• RefSeq Size: 3397 bp
• RefSeq ORF: 1059 bp
• Locus ID: 72795
• UniProt ID: Q8CC21
• Cytogenetics: 11 B2
• Gene Summary: Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed:21278747, PubMed:28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation into the complex III and whose presence is detrimental for its catalytic activity (PubMed:28673544).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) is shorter and lacks an internal segment, as compared to isoform 1.